Hostname: page-component-7479d7b7d-rvbq7 Total loading time: 0 Render date: 2024-07-15T17:14:35.789Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic analysis of the right (3′) end of the rosy locus in Drosophila melanogaster

Published online by Cambridge University Press:  14 April 2009

S. H. Clark ,
A. J. Hilliker  and
A. Chovnick
S. H. Clark
Affiliation:
Department of Molecular and Cell Biology, The University of Connecticut, Storrs, Connecticut 06268
A. J. Hilliker
Affiliation:
Department of Molecular and Cell Biology, The University of Connecticut, Storrs, Connecticut 06268
A. Chovnick
Affiliation:
Department of Molecular and Cell Biology, The University of Connecticut, Storrs, Connecticut 06268

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Prior reports from this laboratory have described the experimental basis for our understanding of the rosy locus (ry: 3–52·0) of Drosophila melanogaster as a bipartite genetic entity consisting of a structural element that codes for the xanthine dehydrogenase (XDH) peptide and a contiguous cis-acting control element immediately to the left of the structural element. Although the left end (5′) of the structural element has been well defined, the right boundary (3′) has been given only casual treatment in our prior reports. In our recent studies of rosy locus expression we have been concerned with the production and identification of mutations in the non-structural regions immediately flanking the structural element. An improved definition of the right end of the structural element is essential to this analysis. In addition to producing a better definition of the right boundary of the structural element, this study produced several phenotypically novel mutations. These mutations were classified initially ascontrol element mutations, but upon analysis were found to map within the rosy structural element. No evidence was obtained for the existence of a control element contiguous with the right end of the structural element.

Type
Research Article
Information
Genetics Research , Volume 47 , Issue 2 , April 1986 , pp. 109 - 116
Copyright
Copyright © Cambridge University Press 1986

References

Chovnick, A., Ballantyne, G. H., Baillie, D. L. & Holm, D. G. (1970). Gene conversion in higher organisms: Halftetrad analysis of recombination within the rosy cistron of Drosophila melanogaster. Genetics 66, 315–329.CrossRefGoogle ScholarPubMed
Chovnick, A., Ballantyne, G. H. & Hoim, D. G. (1971). Studies on gene conversion and its relationship to linked exchange in Drosophila melanogaster. Genetics 69, 179–209.CrossRefGoogle ScholarPubMed
Chovnick, A., Gelbart, W., McCarron, M., Osmond, B., Candido, E. P. M. & Baillie, D. L. (1976). Organization of the rosy locus in Drosophila melanogaster: Evidence for a control element adjacent to the xanthine dehydrogenase structural element. Genetics 84, 233–255.CrossRefGoogle Scholar
Chovnick, A., McCarron, M., Clark, S. H., Hilliker, A. J. & Rushlow, C. A. (1980). Structural and functional organization of a gene in Drosophila melanogaster. In Development and Neurobiology of Drosophila (ed. Siddiqi, O., Babu, P., Hall, L. M. and Hall, J. C.). pp. 3–23. New York: Plenum.CrossRefGoogle Scholar
Clark, S. H., Daniels, S., Rushlow, C. A., Hilliker, A. J. & Chovnick, A. (1984). Tissue-specific and pretranslational character of variants of the rosy locus control element in Drosophila melanogaster. Genetics 108, 953–968.CrossRefGoogle ScholarPubMed
Cote, B., Bender, W., Curtis, D. & Chovnick, A. (1986). Molecular mapping of the rosy locus in Drosphila melanogaster. Genetics (In the Press).Google Scholar
Gelbart, W., McCarron, M. & Chovnick, A. (1976). Extension of the limits of the XDH structural element in Drosophila melanogaster. Genetics 84, 211–232.Google Scholar
Gelbart, W. M., McCarron, M., Pandey, J. & Chovnick, A. (1974). Genetic limits of the xanthine dehydrogenase structural element within the rosy locus in Drosophila melanogaster. Genetics 78, 869–886.Google Scholar
Glassman, E., Karam, J. D. & Keller, E. C. (1962). Differential response to gene dosage experiments involving the two loci which control xanthine dehydrogenase of Drosophila melanogaster. Zeitschrifi für Vererbungslehre 93, 399–403.Google Scholar
Glassman, E. & Mitchell, H. K. (1959). Mutants of Drosophila melanogaster deficient in xanthine dehydrogenase. Genetics 44, 153–162.Google Scholar
Grell, E. H. (1962). The dose effect of ma-1+ and ry + on xanthine dehydrogenase activity in Drosophila melanogaster. Zeitschrft für Vererbungslehre 93, 371–377.Google Scholar
Hilliker, A. J. & Chovnick, A. (1981). Further observations on intragenic recombination in Drosophila melanogaster. Genetical Research 38, 281–296.CrossRefGoogle ScholarPubMed
Hilliker, A. J., Clark, S. H., Chovnick, A. & Gelbart, W. M. (1980). Cytogenetic analysis of the chromosomal region immediately adjacent to the rosy locus in Drosophila melanogaster. Genetics 95, 95–110.CrossRefGoogle Scholar
Lindsley, D. L. & Grell, E. H. (1968). Genetic variations of Drosophila melanogaster. Carnegie Institute Publication Number 627.Google Scholar
McCarron, M. & Chovnick, A. (1981). Induced control mutants at the rosy locus in Drosophila melanogaster. Genetics 97, S70–S71.Google Scholar
McCarron, M., Gelbart, W. & Chovnick, A. (1974). Intracistronic mapping of electrophoretic sites in Drosophila melanogaster: Fidelity of information transfer by gene conversion. Genetics 76, 289–299.CrossRefGoogle ScholarPubMed
McCarron, M., O'Donnell, J., Chovnick, A., Bhullar, B. S., Hewitt, J. & Candido, E. P. M. (1979). Organization of the rosy locus in Drosophila melanogaster: Further evidence in support of a cis-acting control element adjacent to the xanthine dehydrogenase structural element. Genetics 91, 275–293.CrossRefGoogle Scholar
Rushlow, C. A., Bender, W. & Chovnick, A. (1984). Studies on the mechanism of heterochromatic position effect at the rosy locus of Drosophila melanogaster. Genetics 108, 603–615.CrossRefGoogle ScholarPubMed
Rushlow, C. A. & Chovnick, A. (1984). Heterochromatic position effect at the rosy locus of Drosophila melanogaster: Cytological, genetic and biochemical characterization. Genetics 108, 589–602.Google Scholar
Yen, T. T. & Glassman, E. (1965). Electrophoretic variants of xanthine dehydrogenase in Drosophila melanogaster. Genetics 52, 977–981.CrossRefGoogle ScholarPubMed