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Glu-B2, a storage protein locus controlling the D group of LMW glutenin subunits in bread wheat (Triticum aestivum)

Published online by Cambridge University Press:  14 April 2009

Elizabeth A. Jackson ,
Linda M. Holt  and
Peter I. Payne
Elizabeth A. Jackson
Affiliation:
Plant Breeding Institute, Maris Lane, Trumpington, Cambridge CB2 2LQ
Linda M. Holt
Affiliation:
Plant Breeding Institute, Maris Lane, Trumpington, Cambridge CB2 2LQ
Peter I. Payne
Affiliation:
Plant Breeding Institute, Maris Lane, Trumpington, Cambridge CB2 2LQ

Summary

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Genes controlling the synthesis of the D group of low-molecular-weight (LMW) subunits of glutenin occur on the short arms of chromosomes 1B and 1 D. Their position on chromosome 1 B, relative to the storage protein loci Glu-B1 (long arm) and Gli-B1 (short arm), was estimated by analysing the backcross-one progeny of two different crosses. To estimate recombination between the D subunit genes and Gli-B1, half grains were analysed by two-dimensional electrophoresis. The Gli-B1 locus contains genes for the B group of LMW glutenin subunits, γ-gliadins and ω-gliadins although only the latter were made use of in this study to distinguish the parental alleles. Additionally, the complementary half grains were analysed by sodium dodecyl sulphate, polyacrylamide-gel electrophoresis to estimate recombination between Gli-B1 and Glu B1, coding for high-molecular-weight (HMW) glutenin subunits. The D subunit genes occur at a new locus, provisionally defined as Glu-B2, which lies in between Glu-B1 and Gli-B1, 17 cM from the former and 22 cM from the latter. On the basis of previous mapping data involving Gli-B1, it was concluded that the D subunit genes occur close to the nucleolar organizing region and probably on the short-arm satellite, like Gli-B1.

Type
Research Article
Information
Genetics Research , Volume 46 , Issue 1 , August 1985 , pp. 11 - 17
Copyright
Copyright © Cambridge University Press 1985

References

REFERENCES

Bietz, J. A., Shepherd, K. W. & Wall, J. S. (1975). Single-kernel analysis of glutenin: use in wheat genetics and breeding. Cereal Chemistry 52, 513–532.Google Scholar
Brown, J. W. S. & Flavell, R. B. (1981). Fractionation of wheat gliadin subunits by two-dimensional electrophoresis and the role of the group 6 and group 2 chromosomes in gliadin synthesis. Theoretical and Applied Genetics 59, 349–359.CrossRefGoogle Scholar
Galili, G. & Feldman, M. (1983). Genetic control of endosperm proteins in wheat. 2. Variation in high-molecular-weight glutenin and gliadin subunits of Triticum aestivum. Theoretical and Applied Genetics 60, 77–86.CrossRefGoogle Scholar
Galili, G. & Feldman, M. (1984). Mapping of glutenin and gliadin genes located on chromosome 1B of common wheat. Molecular and General Genetics 193, 293–298.CrossRefGoogle Scholar
Holt, L. M., Astin, R. & Payne, P. I. (1981). Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. Part 2. Relative isoelectric points determined by two-dimensional fractionation in polyacrylamide gels. Theoretical and Applied Genetics 60, 237–243.Google Scholar
Jackson, E. A., Holt, L. M. & Payne, P. I. (1983). Characterisation of high-molecular-weight gliadin and low-molecular-weight glutenin subunits of wheat endosperm by two-dimensional electrophoresis and the chromosomal location of their controlling genes. Theoretical and Applied Genetics 66, 29–37.CrossRefGoogle ScholarPubMed
Lawrence, G. J. & Shepherd, K. W. (1980). Variation in glutenin protein subunits of wheat. Australian Journal of Biological Sciences 33, 221–233.CrossRefGoogle Scholar
Lawrence, G. J. & Shepherd, K. W. (1981). Chromosomal location of genes controlling seed proteins in species related to wheat. Theoretical and Applied Genetics 59, 25–31.CrossRefGoogle ScholarPubMed
O'Farrell, P. H. (1975). High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250, 4007–4021.CrossRefGoogle ScholarPubMed
O'Farrell, P. Z., Goodman, H. M. & O'Farrell, P. H. (1977). High resolution two-dimensional electrophoresis of basic as well as acid proteins. Cell 12, 1133–1142.CrossRefGoogle Scholar
Payne, P. I. & Corfield, K. G. (1979). Subunit composition of wheat glutenin proteins, isolated by gel filtration in a dissociating medium. Planta 145, 83–88.CrossRefGoogle Scholar
Payne, P. I., Law, C. N. & Mudd, E. E. (1980). Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theoretical and Applied Genetics 58, 113–120.CrossRefGoogle Scholar
Payne, P. I., Corfield, K. G., Holt, L. M. & Blackman, J. A. (1981). Correlations between the inheritance of certain high-molecular-weight subunits of glutenin and bread-making quality in progenies of six crosses of bread wheat. Journal of the Science of Food and Agriculture 32, 51–60.CrossRefGoogle Scholar
Payne, P. I., Holt, L. M., Worland, A. J. & Law, C. N. (1982). Structural and genetical studies on the high-molecular-weight subunits of wheat glutenin. Part 3. Telocentric mapping of the subunit genes on the long arms of the homoeologous group 1 chromosomes. Theoretical and Applied Genetics 63, 129–138.CrossRefGoogle Scholar
Payne, P. I. & Lawrence, G. J. (1983). Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1, and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Research Communications 11, 29–35.Google Scholar
Payne, P. I., Holt, L. M., Hutchinson, J. & Bennett, M. D. (1984 a). Development and characterisation of a line of bread wheat, Triticum aestivum, which lacks the short-arm satellite of chromosome 1B and the Gli-B1 locus. Theoretical and Applied Genetics 68, 327–334.CrossRefGoogle ScholarPubMed
Payne, P. I., Jackson, E. A., Holt, L. M. & Law, C. N. (1984 b). Genetic linkage between endosperm storage protein genes on each of the short arms of chromosomes 1A and 1B in wheat. Theoretical and Applied Genetics 67, 235–243.CrossRefGoogle ScholarPubMed
Singh, N. K. & Shepherd, K. W. (1984). A new approach to studying the variation and genetic control of disulphide linked endosperm proteins in wheat and rye. In Proc. 2nd Int. Workshop on Wheat Gluten Proteins, pp. 129–136. Wageningen, Holland.Google Scholar
Snape, J. W., Flavell, R. B., O'Dell, M., Hughes, W. G. & Payne, P. I. (1985). Intrachromosomal mapping of the nucleolar organiser region relative to three marker loci on chromosome 1B of wheat (Triticum aestivum). Theoretical and Applied Genetics 69, 263–270.CrossRefGoogle ScholarPubMed
Wall, J. S. (1979). The role of wheat proteins in determining baking quality. In Recent Advances in the Biochemistry of Cereals (ed. Laidman, D. L. and Wyn Jones, R. G.), pp. 275–311. London: Academic Press.Google Scholar