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Herbicide-tolerant tobacco mutants selected in situ and recovered via regeneration from cell culture

Published online by Cambridge University Press:  14 April 2009

David N. Radin  and
Peter S. Carlson
David N. Radin
Affiliation:
Department of Genetics, University of California, Berkeley, CA 94720, U.S.A.
Peter S. Carlson
Affiliation:
Department of Crop and Soil Sciences, Michigan State University, East Lansing, MI 48824, U.S.A.

Summary

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The herbicides Bentazone and Phenmedipharm kill the leaves of intact tobacco plants but do not affect callus cultures. Tolerant mutants were isolated by treating leaves of previously γ-irradiated haploid plants with herbicide then excising and culturing the green herbicide-resistant cell clones on the otherwise yellowed leaves. Among plants subsequently regenerated were a total of ten stable independently isolated mutants. Sexual crosses show these ten represent four Bentazone and two Phenmedipharm loci; all mutants were recessive to wild type.

Type
Research Article
Information
Genetics Research , Volume 32 , Issue 1 , August 1978 , pp. 85 - 89
Copyright
Copyright © Cambridge University Press 1978

References

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