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Heterogeneity of human haptoglobin α chains detected by two-dimensional gel electrophoresis

Published online by Cambridge University Press:  14 April 2009

Huw A. John  and
Ian F. Purdom
Huw A. John
Affiliation:
Department of Genetics, University of Edinburgh, Edinburgh, Scotland
Ian F. Purdom
Affiliation:
Department of Genetics, University of Edinburgh, Edinburgh, Scotland

Summary

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The protein spots representing the haptoglobin α1F, α1S and α2 chains in two-dimensional gels of human plasma samples representative of the six common haptoglobin phenotypes were identified by comparing their position with those of purified haptoglobin and distinguished from other spots in the vicinity by comparison with plasma with undetectably low levels of haptoglobin. Silver staining indicated that the α1F chain was represented by one spot in subtypes 1F-1F, 1F-1S and 1F-2, the α1S chain by three spots in subtypes 1S-1S, 1F-1S and 1S-2 and the α2 chain by six spots in 1F-2, 1S-2 and 2–2

Type
Research Article
Information
Genetics Research , Volume 50 , Issue 1 , August 1987 , pp. 17 - 21
Copyright
Copyright © Cambridge University Press 1987

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