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Identification of the α-galactosidase MEL genes in some populations of Saccharomyces cerevisiae: a new gene MEL11

Published online by Cambridge University Press:  14 April 2009

Gennadi I. Naumov ,
Elena S. Naumova ,
Hilkka Turakainen  and
Matti Korhola
Gennadi I. Naumov
Affiliation:
State Institute for Genetics and Selection of Industrial Microorganisms, I Dorozhnyi 1, Moscow 113545, Russia Department of Biosciences, Division of Genetics, P.O. Box 56 (Viikinkaari 5), FIN-00014University of Helsinki, Finland
Elena S. Naumova
Affiliation:
State Institute for Genetics and Selection of Industrial Microorganisms, I Dorozhnyi 1, Moscow 113545, Russia Department of Biosciences, Division of Genetics, P.O. Box 56 (Viikinkaari 5), FIN-00014University of Helsinki, Finland
Hilkka Turakainen
Affiliation:
Department of Biosciences, Division of Genetics, P.O. Box 56 (Viikinkaari 5), FIN-00014University of Helsinki, Finland
Matti Korhola*
Affiliation:
Primalco Ltd, P.O. Box 350 FIN-00101 Helsinki, Finland
*
*Matti Korhola, Department of Biosciences, Division of General Microbiology, P.O. Box 56 (Viikinkaari 9), FIN-00014 University of Helsinki, Finland. Tel: (358 0) 70859212, Fax: (358 0) 70859262.

Summary

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In this report we mapped a new MEL11 gene and summarize our population studies of the α-galactosidase MEL genes of S. cerevisiae. The unique family of structural MEL genes has undergone rapid translocations to the telomeres of most chromosomes in some specific Saccharomyces cerevisiae populations inhabiting olive oil processing waste (alpechin) and animal intestines. A comparative study of MEL genes in wine, pathogenic and alpechin populations of S.cerevisiae was conducted using genetic hybridization analysis, molecular karyotypingand Southern hybridization with the MEL1 probe. Five polymeric genes for the fermentation of melibiose, MEL3, MEL4, MEL6, MEL7, MEL11, were identified in an alpechin strain CBS 3081. The new MEL11 gene was mapped by tetrad analysis to the left telomeric region of chromosome I. In contrast, in wine and pathogenic populations of S. cerevisiae, MEL genes have been apparently eliminated. Their rare Mel+ strains carry only one of the MEL1, MEL2, or MEL8 genes. One clinical strain YJM273 was heterozygotic on the MEL1 gene; its mell0 allele did not have a sequence of the gene.

Type
Research Article
Information
Genetics Research , Volume 67 , Issue 2 , April 1996 , pp. 101 - 108
Copyright
Copyright © Cambridge University Press 1996

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