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Influence of parental strains on the germination of Phycomyces blakesleeanus zygospores

Published online by Cambridge University Press:  14 April 2009

María José F. Sarabia ,
Arturo P. Eslava  and
María Isabel Alvarez
María José F. Sarabia
Affiliation:
Departamento de Genética, Facultad de Biología, Universidad de Salamanca, 37008-Salamanca, Spain
Arturo P. Eslava*
Affiliation:
Departamento de Genética, Facultad de Biología, Universidad de Salamanca, 37008-Salamanca, Spain
María Isabel Alvarez
Affiliation:
Departamento de Genética, Facultad de Biología, Universidad de Salamanca, 37008-Salamanca, Spain
*
* Corresponding authors.

Summary

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Phycomyces blakesleeanus wild-type NRRL1555( − ), the standard strain, when crossed with UBC21( + ), another wild type, gives zygospores that germinate in 50–60 days. By backcrossing to UBC21 and selecting for shorter dormancy we have isolated a ( − ) strain, A803, and a ( + ) strain A804, which when crossed give zygospores that germinate in 32 days, the shortest dormancy period found in Phycomyces. The same result was obtained when A803 was crossed with UBC21. Zygospore dormancy decreased as the parental strains became more isogenic with UBC21, but the number of zygospores giving germsporangia with viable germspores also decreased to zero in the third backcross. The existence of germspore-killer alleles in the strain UBC21 is postulated. The strains of shortest dormancy can be used as helper strains (Orejas et al. 1985) in sexual crosses. Tetrad analysis of the cross NRRL1554 × S102, a two-factor cross, showed 90% of reciprocal ditypes plus tetratypes in the progeny, indicating that the ( + ) wild-type strain NRRL1554, when crossed with the standard strain, gives regular meiosis and, contrary to current beliefs, may be used in Phycomyces genetic analysis.

Type
Research Article
Information
Genetics Research , Volume 52 , Issue 2 , October 1988 , pp. 91 - 95
Copyright
Copyright © Cambridge University Press 1988

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