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The isolation of mutants affecting ascus development in Neurospora crassa and their analysis by a zygote complementation test

Published online by Cambridge University Press:  14 April 2009

Adrian M. Srb  and
Mary Basl
Adrian M. Srb
Affiliation:
Section of Genetics, Development, and Physiology, Cornell University, Ithaca, New York, U.S.A.
Mary Basl
Affiliation:
Section of Genetics, Development, and Physiology, Cornell University, Ithaca, New York, U.S.A.

Extract

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A large number of mutants that affect the morphology of the ascus of Neurospora crassa has been isolated by means of a screening technique that utilizes the filtration principle to separate colonial type mutants from wild type. In relationships with each other and with wild-type alleles, the mutant genes exhibit zygote dominance and recessiveness. A zygote complementation test for placing the recessive mutants into functional groupings is described. Results obtained by use of the test place the mutants into seven functional groups. Dominance relations in the developing ascus of Neurospora are discussed.

Type
Research Article
Information
Genetics Research , Volume 13 , Issue 3 , June 1969 , pp. 303 - 311
Copyright
Copyright © Cambridge University Press 1969

References

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