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Meiotic recombination in a duplication strain of Aspergillus nidulans

Published online by Cambridge University Press:  14 April 2009

C. Van de Vate
Affiliation:
Department of Molecular Cell Biology, State University of Utrecht, Padualaan 8, Utrecht, The Netherlands
G. J. O. Jansen
Affiliation:
Department of Molecular Cell Biology, State University of Utrecht, Padualaan 8, Utrecht, The Netherlands

Summary

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The (I; II) duplication strain of Aspergillus nidulans contains a terminal segment of chromosome I twice, one copy being in the normal position, and the other one translocated to a tip of chromosome II. We show that in selfed cleistothecia (homozygous for the duplication) crossing-over between the two segments of the duplication followed by the appropriate disjunction and distribution of the meiotic chromosomes, results in segregation of recessive markers in the duplication. Alternative segregation mechanisms like the deletion mechanism responsible for mitotic non-conformity, cannot explain the observations. The average segregation frequency of the marker yA2 is 11%. Using the mathematical model outlined in the Appendix to this paper, we estimated the recombination frequency between the translocation breakpoint and the yA locus from the segregation frequency. The essential feature of the model is the computation of the probabilities of combinations of different tetrad types in quadrivalents. Application of the model leads to the qualitative conclusion that the meiotic recombination frequency within the duplication is increased over the normal level. Elsewhere in the genome the frequencies are either unchanged or decreased. Also in crosses heterozygous for the duplication, an increase by at least a factor of two is found. As judged from the appearance of certain recombinant classes, the quadrivalent frequency in homozygous and heterozygous crosses is 2/3 (random pairing) or slightly higher. The increased recombination is attributed to the same lesions that are responsible for the occurrence of deletions in duplication strains of A. nidulans.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1978

References

REFERENCES

Barratt, K. W., Newmeyer, D., Perkins, D. D. & Garnjobst, L. (1954). Map construction in Neurospora crassa. Advances in Genetics 6, 193.CrossRefGoogle ScholarPubMed
Emerson, R. A. & Rhoades, M. M. (1933). Relation of chromatid crossing over to the upper limit of recombination percentages. The American Naturalist 67, 374377.CrossRefGoogle Scholar
Fincham, J. R. S. & Day, P. R. (1971). Fungal Genetics. Oxford, Edinburgh: Blackwell Scientific Publications.Google Scholar
Papazian, H. P. (1952). The analysis of tetrad data. Genetics 37, 175188.CrossRefGoogle ScholarPubMed
Sybenga, J. (1970). Simultaneous negative and positive interference across the breakpoint in interchange heterozygotes. Genetica 41, 209230.CrossRefGoogle Scholar
Sybenga, J. (1975). Meiotic configurations. Berlin, Heidelberg, New York: Springer Verlag.CrossRefGoogle Scholar
Sybenga, J., Wilms, H. J. & Mulder, A. D. (1973). Genetic length and interference in telo substituted interchange heterozygotes of rye. Chromosoma 42, 95104.CrossRefGoogle ScholarPubMed