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Mutagen specificity among reversions of ultraviolet-induced adenine-1 mutants of Schizosaccharomyces pombe

Published online by Cambridge University Press:  14 April 2009

C. H. Clarke
C. H. Clarke
Affiliation:
M.R.C. Mutagenesis Research Unit, Institute of Animal Genetics, Edinburgh, 9

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Ten U.V.-induced mutants at the adenine-1 locus of Schizosaccharomyces pombe differ not only in their intragenic location but also in their spontaneous, HNO2- and U.V.-induced frequencies of reversion to adenine-independence. Seven mutants reverted with HNO2 and of these only two reverted also with U.V.; the other three did not revert with either HNO2 or U.V.

Genetic analysis of revertants showed that they could be due to suppressor mutations at a locus or loci far from the adenine-1 locus, or to events extremely close to, if not identical with, the original site of mutation. Many distant suppressors were found among spontaneous revertants of some adenine-1 and adenine-7 allles, but such suppressors appear to be less frequent among the five adenine-8 mutants tested. There are large differences between different sites at the same locus in the proportion of their spontaneous revertants due to distant suppressor mutations. Nitrous acid-induced revertants of adenine1-, adenine-7, and adenine-8 mutants were almost all of a type containing no detectable suppressor mutation. In the three cases studied, U.V.-induced revertants could be either predominantly of a suppressor or non-suppressor type, depending on the particular mutant examined.

Striking examples of mutagen specificity were found in which a particular mutant gave mostly suppressor-type revertants with one mutagen, while with another mutagen revertants were mainly of a type containing no detectable suppressor mutation.

Type
Research Article
Information
Genetics Research , Volume 6 , Issue 3 , November 1965 , pp. 433 - 441
Copyright
Copyright © Cambridge University Press 1965

References

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