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A new method of detecting centromere linkage in homothallic yeast

Published online by Cambridge University Press:  14 April 2009

A. P. James
A. P. James
Affiliation:
Division of Biological Sciences, National Research Council of Canada, Ottawa, CanadaK1A 0R6

Summary

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A method is described for detecting centromere linkage in Saccharomyces cerevisiae using a simple monohybrid for the locus in question. The method utilizes the two-spored asci produced by a normal homothallic diploid and depends upon a selective elimination of specific chromatids during spore formation. The pattern of selective elimination was not detected in a heterothallic strain but was found to be a dominant trait in an inter-strain cross. It was shown that gross chromosome aberrations are probably not responsible for the two-spored asci produced by either the heterothallic or homothallic strains.

Type
Research Article
Information
Genetics Research , Volume 23 , Issue 2 , April 1974 , pp. 201 - 206
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

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