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The organization and composition of the ribosomal RNA gene non-transcribed spacer of D. busckii is unique among the drosophilids

Published online by Cambridge University Press:  14 April 2009

James M. Slavicek  and
Hallie M. Krider
James M. Slavicek
Affiliation:
Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut, 06268
Hallie M. Krider
Affiliation:
Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut, 06268

Summary

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Several ribosomal RNA (rRNA) genes from D. busckii were cloned and characterized. The prominent repeat classes have lengths of 12·8 and 13·6 kb and lack 28S introns. rRNA genes were cloned containing 28S insertions which exhibit heterogeneity in size and sequence. The non-transcribed spacer (NTS) contains two regions composed of different repeated sequences that exhibit pronounced instability in HB 101. NTS region II, centrally located within the NTS, contains predominately 11 or 16 HincII generated 160 bp repeats. NTS region III, next to the 18S gene, contains repeats that are variable in number, and are either heterogeneous in length or are dispersed within unique sequences. The organization and composition of the rRNA gene NTS of D. busckii is different in comparison to the NTSs of other drosophilids. In addition, the pronounced instability of two different NTS regions is unique in comparison to all other cloned rRNA genes.

Type
Research Article
Information
Genetics Research , Volume 50 , Issue 3 , December 1987 , pp. 173 - 180
Copyright
Copyright © Cambridge University Press 1987

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