Hostname: page-component-77c89778f8-9q27g Total loading time: 0 Render date: 2024-07-16T23:07:13.087Z Has data issue: false hasContentIssue false
  • English
  • Français

Phenotypic characterization of R-factor tetracycline resistance determinants

Published online by Cambridge University Press:  14 April 2009

T. J. Foster  and
Áine Walsh
T. J. Foster
Affiliation:
Bacteriology Department, Trinity College, Dublin 2, Irish Republic
Áine Walsh
Affiliation:
Bacteriology Department, Trinity College, Dublin 2, Irish Republic

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The tetracycline-resistance determinants of R-factors from different compatibility groups have been tested in Escherichia coli K12 and phenotypically classified into two major classes. Class I determinants confer high-level resistance to tetracycline (> 100 μg/ml) and moderate resistance to minocycline (5–25 μg/ml) while those of Class II gave moderate resistance to tetracycline (50–70 μg/ml) and low resistance to minocycline. Each class was subdivided because of variation in resistance profiles and in the abilities of tetracycline and minocycline to induce increased resistance. Strains carrying two compatible Tetr R-factors of the same or different phenotypic groups did not show increased tetracycline resistance.

Type
Research Article
Information
Genetics Research , Volume 24 , Issue 3 , December 1974 , pp. 333 - 343
Copyright
Copyright © Cambridge University Press 1974

References

REFERENCES

Chabbert, Y. A., Scavizzi, M. R., Whitchitz, J. L., Gerbaud, G. R. & Bouanchaud, D. H. (1972). Incompatibility groups and the classification of fi − resistance factors. Journal of Bacteriology 112, 666–675.CrossRefGoogle ScholarPubMed
Egawa, R. & Hirota, Y. (1962). Inhibition of fertility by multiple drug resistance factor in Escherichia coli K12. Japanese Journal of Genetics 37, 66–69.Google Scholar
Foster, T. J. & Shaw, W. V. (1973). Chloramphenicol acetyltransferases specified by fi − R factors. Antimicrobial Agents and Chemotherapy 3, 99–104.CrossRefGoogle Scholar
Franklin, T. J. (1967). Resistance of Escherchia coli to tetracyclines. Changes in permeability to tetracyclines in Escherichia coli bearing transmissible resistance factors. Biochemical Journal 105, 371–378.CrossRefGoogle Scholar
Franklin, T. J. & Foster, S. J. (1971). Effect of osmotic shock on tetracycline resistance in Escherichia coli bearing an R factor. Biochemical Journal 121, 287–292.CrossRefGoogle ScholarPubMed
Franklin, T. J. & Godfrey, A. (1965). Resistance of Escherichia coli to tetracyclines. Biochemical Journal 94, 54–60.CrossRefGoogle ScholarPubMed
Franklin, T. J. & Higginson, B. (1970). Active accumulation of tetracycline by Escherichia coli. Biochemical Journal 116, 287–297.CrossRefGoogle ScholarPubMed
Grinsted, J., Saunders, J. R., Ingram, L. C., Sykes, R. B. & Richmond, M. H. (1972). Properties of an R factor which originated in Pseudomonas aeruginosa 1822. Journal of Bacteriology 110, 529–537.CrossRefGoogle Scholar
Hedges, R. W. (1972). Phenotypic characterization of fi − R factors determining the restriction and modification hsp II specificity. Molecular and General Genetics 115, 225–283.CrossRefGoogle ScholarPubMed
Izaki, K. & Arima, K. (1963). Disappearance of oxytetracycline accumulation in the cells of multiple drug-resistance Escherichia coli. Nature 200, 384–385.CrossRefGoogle ScholarPubMed
Jarolman, H., Hewel, D. & Kain, E. (1970). Activity of minocycline against R-factor-carrying Enterobacteriaceae. Infection and Immunity 1, 321–326.CrossRefGoogle Scholar
Lawn, A. M., Meynell, E., Meynell, G. G. & Datta, N. (1967). Sex pili and the classification of sex factors in the Enterobacteriaceae. Nature 216, 343–346.CrossRefGoogle ScholarPubMed
Nordstrom, K., Ingram, L. C. & Lundback, A. (1972). Mutations in R factors of Escherichia coli causing an increased number of R factor copies per chromosome. Journal of Bacteriology 110, 562–569.CrossRefGoogle Scholar
Reynard, A. M. & Nellis, L. F. (1972). Uptake of tetracycline by Escherichia coli: lack of binding of tetracycline to the uptake system. Biochemical and Biophysical Research Communications 48, 1129–1132.CrossRefGoogle Scholar
Robertson, J. M. & Reeve, E. C. R. (1972). Analysis of the resistance mediated by several R-factors to tetracycline and minocycline. Genetical Research 20, 239–252.CrossRefGoogle ScholarPubMed
Romero, E. & Meynell, E. (1969). Covert fi − R factors in fi + R+ strains of bacteria. Journal of Bacteriology 97, 780–786.CrossRefGoogle ScholarPubMed
Scavizzi, M. R. (1972). Existence de deux caractères transférables de résistance aux cyclines. Annales de I'Institut Pasteur 122, 3–17.Google Scholar