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Plasmid-mediated transmission of chromosomal genes in Pseudomonas glycinea*

Published online by Cambridge University Press:  14 April 2009

George H. Lacy
Affiliation:
Department of Plant Pathology, University of California, Riverside, California 92502, U.S.A.
John V. Leary
Affiliation:
Department of Plant Pathology, University of California, Riverside, California 92502, U.S.A.

Summary

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Complementing diauxotrophic mutants of P. glycinea were mated in combination in which one or both mutants contained a resistance plasmid. The transfer of chromosmal markers was scored by the appearance of prototrophy at one or more of the auxotrophic loci. The resistance plasmid RP1 was equally or slightly more efficient than R6886 in the transmission of chromosomal genes.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1976

References

REFERENCES

Carhart, G. F. & Hegeman, G. D. (1974). Development of an improved method of selection for auxotrophic mutants of Pseudomonas (Abstract). Genetics 77, s10.Google Scholar
Coplin, D. L., Sequeira, L. & Hanson, R. S. (1974). Pseudomonas solanacearum: Virulence of biochemical mutants. Canadian Journal of Microbiology 20, 519529.CrossRefGoogle ScholarPubMed
Curtiss, R. III (1969). Bacterial conjugation. Annual Review of Microbiology 23, 69136.CrossRefGoogle ScholarPubMed
Datta, N. & Hedges, R. W. (1971). Compatibility groups among fi R factors. Nature 234, 222223.CrossRefGoogle ScholarPubMed
Delic, V., Hopwood, D. A. & Friend, E. J. (1970). Mutagenesis by N-methyl-N'-nitro-N'-nitrosoguanidine (NTG) in Streptomyces coelicolor. Mutation Research 9, 167182.CrossRefGoogle Scholar
Doudoroff, M. & Palleroni, N. J. (1974). Genus I. Pseudomonas Migula 1894, 237 Nom. cons. Opin. 5, Jud. Comm, 1952, 121. In Bergey's Manual of Determinative Bacteriology, 8th ed. (ed. Buchanan, R. E. & Gibbon, N. E.). Baltimore: Williams & Wilkins.Google Scholar
Garrett, C. M. E. & Crosse, J. E. (1963). Observations on lysogeny in the plant pathogens Pseudonomas mors-prunorum and Ps. syringae. Journal of Applied Bacteriology 26, 2734.CrossRefGoogle Scholar
Holliday, R. (1956). A new method for the identification of biochemical mutants of microorganisms. Nature 178, 987.CrossRefGoogle Scholar
Holloway, B. W. (1969). Genetics of Pseudomonas. Bacteriological Reviews 33, 419443.CrossRefGoogle ScholarPubMed
Holloway, B. W., Krishnapillai, V. & Stanisich, V. A. (1971). Pseudomonas genetics. Annual Review of Genetics 5, 425446.CrossRefGoogle ScholarPubMed
Lacy, G. H. (1975). Genetics of Pseudomonas glycinea Coerper. Ph.D. dissertation, University of California, Riverside.Google Scholar
Lacy, G. H. & Leary, J. V. (1975). Transfer of antibiotic resistance plasmid RPl into Pseudomonas glydnea and Pseudomonas phaseolicola in vitro and in planta. Journal of General Microbiology 88, 4957.CrossRefGoogle Scholar
Liu, S. C. Y. (1973). Inter-specific transformation between Pseudomonas syringae and P. solanacearum (Abstract). 2nd International Congress of Plant Pathology,Minneapolis, Minnesota,5–12 09.Google Scholar
Loutit, J. S. (1969). Investigation of the mating system of Pseudomonas aeruginosa strain 1. IV. Mapping of distal markers. Genetical Research 13, 9198.CrossRefGoogle ScholarPubMed
Nishimura, A., Nishimbura, Y. & Caro, L. (1973). Isolation of Hfr strains from R+ and Col V2+ strains of Escherichia coli and derivation of an R'lac factor by transduction. Journal of Bacteriology 116, 11071112.CrossRefGoogle Scholar
Okabe, N. & Goto, M. (1955). Studies on Pseud, solanacearum X. Genetic change of the bacterial strains induced by the temperate Phage T-c200. Report of the Faculty of Agriculture, Shizuoka University 5, 5762.Google Scholar
Ornston, L. N., Ornston, M. K. & Chou, G. (1969). Isolation of spontaneous mutant strains of Pseudomonas putida. Biochemical and Biophysical Research Communications 36, 179184.CrossRefGoogle ScholarPubMed
Panopoulos, N. J., Guimaraes, W. V., Cho, J. J. & Schboth, M. N. (1975). Conjugative transfer of Pseudomonas aeruginosa R factors to plant pathogenic Pseudomonas spp. Phytopathology 65, 380388.Google Scholar
Stanisich, V. A. & Holloway, B. W. (1969). Conjugation in Pseudomonas aeruginosa. Genetics 61, 327339.CrossRefGoogle ScholarPubMed
Stanisich, V. A. & Holloway, B. W. (1971). Chromosome transfer in Pseudomonas aeruginosa mediated by R-factors. Genetical Research 17, 169172.CrossRefGoogle ScholarPubMed
Twiddy, W. & Liu, S. C. Y. (1972). Intraspecific transformation of Pseudomonas syringae (Abstract). Phytopathology 62, 794.Google Scholar