Hostname: page-component-7479d7b7d-rvbq7 Total loading time: 0 Render date: 2024-07-12T01:18:56.889Z Has data issue: false hasContentIssue false
  • English
  • Français

Pleiotropy of p-fluorophenylalanine-resistant and antibiotic hypersensitive mutants of Pseudomonas aeruginosa

Published online by Cambridge University Press:  14 April 2009

N. W. Dunn  and
B. W. Holloway
N. W. Dunn
Affiliation:
Department of Genetics, Monash University, Clayton, Victoria 3168, Australia
B. W. Holloway
Affiliation:
Department of Genetics, Monash University, Clayton, Victoria 3168, Australia

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Mutants of Pseudomonas aeruginosa isolated as being resistant to p-fluorophenylalanine (FPA-r) are pleiotropic, showing patterns of phenotypic alteration of host-controlled modification, growth rate at 37 and 43 °C, ability to grow on minimal medium, response to antibiotics and ribosomal characteristics. Alterations in host-controlled modification are also found in FPA-r mutants of Escherichia coli B.

The pleiotropy arising in these FPA-r mutants of P. aeruginosa appears to result from alterations in the specificity of protein synthesis. Phenotypic variations in host-controlled modification of the type found in the FPA-r mutants may provide a method for the detection of suppressor mutations which act by miscoding.

Type
Research Article
Information
Genetics Research , Volume 18 , Issue 2 , October 1971 , pp. 185 - 197
Copyright
Copyright © Cambridge University Press 1971

References

REFERENCES

Apirion, D. (1966). Altered ribosomes in a suppressor strain of Escherichia coli. Journal of Molecular Biology 16, 285–301.CrossRefGoogle Scholar
Apirion, D. (1967). Three genes that affect Escherichia coli ribosomes. Journal of Molecular Biology 30, 255–275.Google ScholarPubMed
Bruno, G. A. & Christian, J. E. (1961). Determination of carbon-14 in aqueous bicarbonate solutions by liquid scintillation counting techniques. Analytical Chemistry 33, 1216–1218.CrossRefGoogle Scholar
Davies, J. (1966). Streptomycin and the genetic code. Cold Spring Harbor Symposium on Quantitative Biology 31, 665–670.CrossRefGoogle ScholarPubMed
Dussoix, D. & Arber, W. (1962). Host specificity of DNA produced by Escherichia coli. II. Control over acceptance of DNA from infecting phage λ. Journal of Molecular Biology 5, 37–49.CrossRefGoogle ScholarPubMed
von Ehrenstein, G. & Lipmann, F. (1961). Experiments on haemoglorin synthesis. Proceedings of the National Academy of Sciences, U.S.A. 47, 941–950.CrossRefGoogle Scholar
Fangman, W. L. & Neidhardt, F. C. (1964). Demonstration of an altered amino-acyl ribonucleic acid synthetase in a mutant of Escherichia coli. Journal of Biological Chemistry 239, 1839–1843.CrossRefGoogle Scholar
Fargie, B. & Holloway, B. W. (1965). Absence of clustering of functionally related genes in Pseudomonas aeruginosa. Genetical Research 6, 284–299.CrossRefGoogle ScholarPubMed
Gartner, T. K. & Orias, E. (1966). Effects of mutation to streptomycin resistance on the rate of translocation of mutant genetic information. Journal of Bacteriology 91, 1021–1028.CrossRefGoogle Scholar
Gartner, T. K., Orias, E., Lennan, J. E., Beeson, J. & Reid, P. J. (1969). The molecular basis of suppression in an ochre suppressor strain possessing altered ribosomes. Proceedings of the National Academy of Sciences, U.S.A. 62, 946–951.CrossRefGoogle Scholar
Holloway, B. W. (1965). Variations in restriction and modification of bacteriophage following increase in growth temperature of Pseudomonas aeruginosa. Virology 25, 634–642.CrossRefGoogle ScholarPubMed
Holloway, B. W. (1969). Genetics of Pseudomonas. Bacteriological Reviews 33, 419–443.CrossRefGoogle ScholarPubMed
Holloway, B. W., Egan, J. B. & Monk, M. (1960). Lysogeny in Pseudomonas aeruginosa. Australian Journal of Experimental Biology and Medical Science 38, 321–330.CrossRefGoogle ScholarPubMed
Holloway, B. W. & Fargie, B. (1960). Fertility factors and genetic linkage in Pseudomonas aeruginosa. Journal of Bacteriology 80, 362–368.CrossRefGoogle ScholarPubMed
Holloway, B. W., Khishnapillai, V. & Stanisich, V. (1971). Pseudomonas genetics. Annual Review of Genetics (in the Press).CrossRefGoogle Scholar
Holloway, B. W. & van de Putte, P. (1968). Lysogeny and bacterial recombination. In Replication and Recombination of Genetical Material (ed. Peacock, W. J. and Brock, R. D.), pp. 175–183. Australian Academy of Science.Google Scholar
Isaac, J. & Holloway, B. W. (1968). Control of pyrimidine biosynthesis in Pseudomonas aeruginosa. Journal of Bacteriology 96, 1732–1741.CrossRefGoogle ScholarPubMed
Kuwano, M., Ishikawa, M. & Endo, H. (1968). Su-II-specific restriction of amber suppression by mutation to streptomycin resistance. Journal of Molecular Biology 33, 513–516.CrossRefGoogle ScholarPubMed
Lederberg, S. (1957). Suppression of the multiplication of heterologous bacteriophages in lysogenic bacteria. Virology 3, 496–513.CrossRefGoogle ScholarPubMed
Lederberg, E. M., Cavalli-Sforza, L. & Lederberg, J. (1964). Interaction of streptomycin and a suppressor for galactose fermentation in E. coli K12. Proceedings of the National Academy of Sciences, U.S.A. 51, 678–682.CrossRefGoogle Scholar
Lipmann, F. (1969). Polypeptide chain elongation in protein biosynthesis. Science, New York 164, 1024–1031.CrossRefGoogle ScholarPubMed
Nishizuka, Y. & Lipmann, F. (1966). Comparison of guanisine triphosphate split and polypeptide synthesis with a purified E. coli system. Proceedings of the National Academy of Sciences, U.S.A. 55, 212–219.CrossRefGoogle Scholar
Otsuji, N. & Aono, H. (1968). Effect of mutation to streptomycin resistance on amber suppressor genes. Journal of Bacteriology 96, 43–50.CrossRefGoogle ScholarPubMed
Rolfe, B. & Holloway, B. W. (1968). Genetic control of DNA specificity in Pseudomonas aeruginosa. Genetical Research, Cambridge 12, 99–102.CrossRefGoogle ScholarPubMed
Stanisich, V. & Holloway, B. W. (1969). Conjugation in Pseudomonas aeruginosa. Genetics 61, 327–339.CrossRefGoogle ScholarPubMed
Waltho, J. A. & Holloway, B. W. (1966). Suppression of fluorophenylalanine resistance by mutation to streptomycin resistance in Pseudomonas aeruginosa. Journal of Bacteriology 92, 35–42.CrossRefGoogle ScholarPubMed
Waltho, J. A. (1968). Genetic aspects of enzyme regulation in Pseudomonas aeruginosa. Ph.D. thesis, University of Melbourne.Google Scholar
Wood, W. B. (1966). Host specificity of DNA produced by Escherichia coli; bacterial mutations affecting restriction and modification of DNA. Journal of Molecular Biology 16, 118–133.CrossRefGoogle ScholarPubMed