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RP4 Fertility Variants in Acinetobacter calcoaceticus

Published online by Cambridge University Press:  14 April 2009

K. J. Towner  and
A. Vivian
K. J. Towner
Affiliation:
School of Biological Sciences, Thames Polytechnic, London SE18 6PF
A. Vivian
Affiliation:
School of Biological Sciences, Thames Polytechnic, London SE18 6PF

Summary

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The resistance plasmid RP4 can be used to mediate conjugation in Acinetobacter calcoaceticus. Transfer of chromosomal genes occurs only on solid surfaces and not in liquid matings. Two RP4-carrying donor strains which donate the chromosome in different directions from different origins have been isolated. As a result it is possible to demonstrate that the linkage map of A. calcoaceticus is circular.

The fate of RP4 in recombinants was examined. Although frequent loss of the complete plasmid was detected, segregation of plasmid antibiotic resistances was not observed.

A possible mechanism by which RP4 could interact with the A. calcoaceticus chromosome is discussed.

Type
Research Article
Information
Genetics Research , Volume 28 , Issue 3 , December 1976 , pp. 301 - 306
Copyright
Copyright © Cambridge University Press 1976

References

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