Hostname: page-component-7bb8b95d7b-fmk2r Total loading time: 0 Render date: 2024-09-18T08:12:48.081Z Has data issue: false hasContentIssue false
  • English
  • Français

Search for ribosomal mutants in Podospora anserina: genetic analysis of cold-sensitive mutants

Published online by Cambridge University Press:  14 April 2009

Marguerite Picard-Bennoun  and
Denise Le Coze
Marguerite Picard-Bennoun
Affiliation:
Laboratoire de Génétique de l' Université Paris-Sud, Centre d'ORSAY (Laboratoire associé au CNRS n° 86) et Laboratoire de Génétique de l'Université Bordeaux II Bâtiment 400, 91405 Orsay, France
Denise Le Coze
Affiliation:
Laboratoire de Génétique de l' Université Paris-Sud, Centre d'ORSAY (Laboratoire associé au CNRS n° 86) et Laboratoire de Génétique de l'Université Bordeaux II Bâtiment 400, 91405 Orsay, France

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Twenty-four cold-sensitive (prototrophic) mutants were isolated after UV mutagenesis of protoplasts of the fungus Podospora anserina. Genetic analysis of these mutants was performed in order to detect those among them which were most likely to be impaired in translational fidelity. The 24 mutations belonged to 24 different genes. One half of the mutants were pleiotropic and displayed an altered phenotype: growth rate at the permissive temperature, germination of the spores, fertility and/or sporulation. Nine mutants differed from wild-type in their resistance levels to cycloheximide, trichodermin and/or paromomycin. Several mutations were linked to known ribosomal loci. Two mutations behaved like informational antisuppressors: one is allelic to the previously described As3 gene and the other one defines a new antisuppressor gene, AS6.

Type
Research Article
Information
Genetics Research , Volume 36 , Issue 3 , December 1980 , pp. 289 - 297
Copyright
Copyright © Cambridge University Press 1980

References

REFERENCES

Bayliss, F. T. & Ingraham, J-L. (1974). Mutation in Saccharomyces cerevisiae conferring streptomycin and cold-sensitivity by affecting ribosome formation and function. Journal of Bacteriology 118, 319–328.CrossRefGoogle ScholarPubMed
Begueret, J., Perrot, M. & Crouzet, M. (1977). Ribosomal proteins in the fungus Podospora anserina: evidence for an electrophoretically altered 60S protein in a cycloheximide resistant mutant. Molecular and General Genetics 156, 141–144.CrossRefGoogle Scholar
Begueret, J. & Picard-Bennoun, M. (1979). Les ribosomes des eucaryotes inférieurs. Biochimie 61, VII–XVIII.Google Scholar
Belcour, L. (1975). Cytoplasmic mutations isolated from protoplasts of Podospora anserina. Genetical Research 25, 155–161.CrossRefGoogle ScholarPubMed
Bollen, A., Cabezon, T., Topisirovic, L., De Wilde, M., Villarroel, R. & Herzog, A. (1976). Ribosomal mutations interfering with translational fidelity. In Ribosomes and RNA Metabolism (ed. Zelinka, and Balan, ), pp. 55–66. Bratislava: Publ. House Slovak Acad. of Sciences.Google Scholar
Bryant, R. E. & Sypherd, P. S. (1974). Genetic analysis of cold-sensitive ribosome maturation mutants of E. coli. Journal of Bacteriology 117, 1082–1092.CrossRefGoogle Scholar
Coppin-Raynal, E. (1977). Ribosomal suppressors and antisuppressors in Podospora anserina: resistance to cycloheximide. Journal of Bacteriology 131, 876–883.CrossRefGoogle ScholarPubMed
Coppin-Raynal, E. (1978). Mise en évidence de la nature ribosomique de mutations suppresseurs et antisuppresseurs chez Podospora anserina. Thèse de 3ème Cycle. Université de Paris XI, Centre d'Orsay, France.Google Scholar
Crouzet, M. & Begueret, J. (1978). Cold-sensitivity of a double mutant strain combining two ribosomal mutations in the ascomycete Podospora anserina. Molecular and General Genetics 165, 283–288.CrossRefGoogle ScholarPubMed
Crouzet, M., Perrot, M., Nogueira, M. & Begueret, J. (1978). Genetic and biochemical analysis of cycloheximide resistance in the fungus Podospora anserina. Biochemical Genetics 16, 271–286.CrossRefGoogle ScholarPubMed
Dequard, M., Couderc, J-L., Legrain, P., Belcour, L. & Picard-Bennoun, M. (1980). Search for ribosomal mutants in Podospora anserina: genetic analysis of mutants resistant to paromomycin. Biochemical Genetics 18, 263–280.CrossRefGoogle ScholarPubMed
De Wilde, M., Cabezon, T., Herzog, A. & Bollen, A. (1977). Apports de la génétique à la connaissance du ribosome bactérien. Biochimie 59, 125–140.CrossRefGoogle Scholar
Esser, K. (1969). An introduction to Podospora anserina. Neurospora Newsletter 15, 27–30.Google Scholar
Falke, E. W. & Wright, T. R. F. (1975). Cold-sensitive mutants of Drosophila melanogaster defective in ribosome assembly. Genetics 81, 655–682.CrossRefGoogle ScholarPubMed
Gerlach, W. L. (1975). Genetic properties of some amberochre super-suppressors in Saccharomyces cerevisiae. Molecular and General Genetics 138, 58–63.CrossRefGoogle Scholar
Gerlach, W. L. (1976). Mutation properties of sup P amberochre super-suppressors in Saccharomyces cerevisiae. Molecular and General Genetics 144, 213–215.CrossRefGoogle Scholar
Gorini, L. (1974). Streptomycin and misreading of the genetic code. In Ribosomes (ed. Nomura, M., Tissières, A. and Lengyel, P.), pp. 791–803. New York: Cold Spring Harbor Laboratory.Google Scholar
Guthrie, C., Nashimoto, H. & Nomura, M. (1969). Structure and function of E. coli ribosomes. VIII Cold-sensitive mutants defective in ribosome assembly. Proceedings of the National Academy of Sciences of United States of America 63, 384–391.CrossRefGoogle Scholar
Hartwell, L., McLaughlin, C. & Warner, J. R. (1970). Identification of ten genes that control ribosome formation in yeast. Molecular and General Genetics 109, 42–56.CrossRefGoogle ScholarPubMed
Hawthorne, D. C. & Leupold, U. (1974). Suppressor mutations in yeast. Current Topics in Microbiology and Immunology 64, 1–47.CrossRefGoogle ScholarPubMed
Jaskunas, S. R., Nomura, M. & Davies, J. (1974). Genetics of bacterial ribosomes. In Ribosomes (ed. Nomura, M., Tissières, A. and Lengyel, P.), pp. 333–368. New York: Cold Spring Harbor Laboratory.Google Scholar
Kohli, J., Altruda, F., Kwong, T., Rafalski, A., Wetzel, R., Soll, D., Wahl, G. & Leupold, U. (1980). Nonsense suppressor transfer RNAs in Schizosaccharomyces pombe. In Transfer RNA. New York: Cold Spring Harbor Laboratory. (In the Press.)Google Scholar
MacCready, S. & Cox, B. S. (1973). Antisuppressors in yeast. Molecular and General Genetics 124, 305–320.CrossRefGoogle Scholar
McLaughlin, C. S. (1974). Yeast ribosomes: genetics. In Ribosomes (ed. Nomura, M., Tissières, A. and Lengyel, P.), pp. 815–827. New York: Cold Spring Harbor Laboratory.Google Scholar
Monnot, F. (1953). Sur la possibilité de réaliser des croisements réciproques chez l'Ascomycète Podospora anserina. Comptes rendus de l' Académie des Sciences (Paris) 236, 2263.Google Scholar
Palmer, E. & Wilhelm, J. M. (1978). Mistranslation in an eucaryotic organism. Cell 13, 329–334.CrossRefGoogle Scholar
Palmer, E., Wilhelm, J. M. & Sherman, F. (1979). Phenotypic suppression of nonsense mutants in yeast by aminoglycoside antibiotics. Nature 277, 148–149.CrossRefGoogle ScholarPubMed
Picard, M. (1971). Genetic evidence for a polycistronic unit of transcription in the complex ‘14’ in Podospora anserina. I. Genetic and complementation maps. Molecular and General Genetics 111, 35–50.CrossRefGoogle ScholarPubMed
Picard, M. (1973). Genetic evidence for a polycistronic unit of transcription in the complex locus ‘14’ in Podospora anserina. II. Genetic analysis of informational suppressors. Genetical Research 21, 1–15.CrossRefGoogle Scholar
Picard-Bennoun, M. (1976). Genetic evidence for ribosomal antisuppressors in Podospora anserina. Molecular and General Genetics 147, 299–306.CrossRefGoogle ScholarPubMed
Picard-Bennoun, M. & Coppin-Raynal, E. (1977). Une fonction ribosomique essentielle: le contrôle de la fidélité de la traduction. Physiologie Végétale 15, 481–489.Google Scholar
Piepersberg, W., Geyl, D., Hummel, H. & Bock, A. (1980). Physiology of ribosomal mutants. Physiology and biochemistry of bacterial ribosomal mutants. In Genetics and evolution of transcriptional and translational apparatus. Tokio: Kodansha Scientific. (In the Press.)Google Scholar
Rizet, G. & Engelmann, C. (1949). Contribution à l'étude génétique d'un ascomycète tétrasporé: Podospora anserina. Revue de cytologie et de biologie végétales 11, 201–304.Google Scholar
Roberts, T., Martinelli, S. & Claudio, Scazzocchio (1979). Allele specific, gene unspecific suppressors in Aspergillus nidulans. Molecular and General Genetics 177, 57–64.CrossRefGoogle ScholarPubMed
Schlitt, S. C. & Russell, P. J. (1974). Neurospora crassa cytoplasmic ribosomes: isolation and characterization of a cold-sensitive mutant defective in ribosome biosynthesis. Journal of Bacteriology 120, 666–671.CrossRefGoogle ScholarPubMed
Singh, A., Ursic, D. & Davies, J. (1979). Phenotypic suppression and misreading in Saccharomyces cerevisiae. Nature 277, 146–148.CrossRefGoogle Scholar
Thuriaux, P., Minet, M., Hofer, F. & Leupold, U. (1975). Genetic analysis of antisuppressor mutants in the fission yeast Schizosaccharomyces pombe. Molecular and General Genetics 142, 251–261.CrossRefGoogle Scholar
Ursic, D. & Davies, J. (1979). A cold-sensitive mutant of Saccharomyces cerevisiae defective in ribosome processing. Molecular and General Genetics 175, 313–323.CrossRefGoogle ScholarPubMed
Waldron, C. & Roberts, C. F. (1974 a). Cold-sensitive mutants in Aspergillus nidulans. I. Isolation and general characterization. Molecular and General Genetics 134, 99–113.CrossRefGoogle Scholar
Waldron, C. & Roberts, C. F. (1974 b). Cold-sensitive mutants in Aspergillus nidulans. II. Mutations affecting ribosome production. Molecular and General Genetics 134, 115–132.CrossRefGoogle ScholarPubMed
Wilhelm, J. M., Pettitt, S. E. & Jessop, J. J. (1978 a). Aminoglycoside antibiotics and eukaryotic protein synthesis: structure-function relationships in the stimulation of misreading with a wheat embryo system. Biochemistry 17, 1143–1149.CrossRefGoogle ScholarPubMed
Wilhelm, J. M., Jessop, J. J. & Pettitt, S. E. (1978 b). Aminoglycoside antibiotics and eukaryotic protein synthesis: stimulation of errors in the translation of natural messengers in extracts of cultured human cells. Biochemistry 17, 1149–1153.CrossRefGoogle ScholarPubMed
Zickler, D. & Simonet, J. M. (1980). Identification of gene-controlled steps of ascospore development in Podospora anserina. Experimental Mycologia (In the Press.)CrossRefGoogle Scholar