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Selection for conditional lethals: A general negative selective system for Schizophyllum commune

Published online by Cambridge University Press:  14 April 2009

Miriam Rich Anderson  and
Carol S. Deppe
Miriam Rich Anderson
Affiliation:
Department of Genetics and Cell Biology, 250 Bio Science Center, University of Minnesota, Saint Paul, Minnesota 55108, U.S.A.
Carol S. Deppe
Affiliation:
Department of Genetics and Cell Biology, 250 Bio Science Center, University of Minnesota, Saint Paul, Minnesota 55108, U.S.A.

Summary

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This paper describes a method for selecting for conditional lethal mutations in the filamentous fungus Schizophyllum commune. The method is, to our knowledge, the only successful application of filtration enrichment to Basidiomycetes. In addition, it is far more powerful than filtration enrichment methods reported previously for any fungus; it permits recovery of up to 80% auxotrophs starting with material untreated with any mutagen.

Type
Short Paper
Information
Genetics Research , Volume 29 , Issue 1 , February 1977 , pp. 93 - 96
Copyright
Copyright © Cambridge University Press 1977

References

REFERENCES

Fries, N. (1947). Experiments with different methods of isolating physiological mutations of filamentous fungi. Nature 159, 199.CrossRefGoogle ScholarPubMed
Ostlund, M. L., Beck, R. J., Anderson, M. R. & Deppe, C. S. Biochemical genetics of Schizophyllum commune. (In preparation.)Google Scholar
Raper, J. R. & Hoffman, R. M. (1974). Schizophyllum commune. Handbook of Genetics 1, 597–626.Google Scholar
Woodward, V. W., De Zeeuw, J. R. & Srb, A. M. (1954). The separation and isolation of particular biochemical mutants of Neurospora by differential germination of conidia, followed by filtration and selective plating. Proceedings of the National Academy of Sciences, U.S.A. 40, 192–200.CrossRefGoogle ScholarPubMed