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The sequence of four structural and two regulatory methionine genes in the Salmonella typhimurium linkage map

Published online by Cambridge University Press:  14 April 2009

P. D. Ayling  and
K. F. Chater
P. D. Ayling
Affiliation:
Department of Genetics, University of Birmingham, England
K. F. Chater
Affiliation:
Department of Genetics, University of Birmingham, England

Extract

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Three-point transduction analysis was employed to determine the sequence of four structural and two regulatory methionine genes (metA, B, F, H and metI, J, respectively) on two apparently adjacent transduction fragments in Salmonella typhimurium. These fragments were subsequently orientated with respect to each other and the rest of the linkage map by the analysis of recombinants obtained in conjugation experiments. The following clockwise sequence of genes is proposed: -metJ-metB-metF-argF-thi-purD-metA-metI-metH-. The bearing of these results on the regulation of the metB–F and metA–H clusters is discussed.

Type
Research Article
Information
Genetics Research , Volume 12 , Issue 3 , December 1968 , pp. 341 - 354
Copyright
Copyright © Cambridge University Press 1968

References

REFERENCES

Armstrong, F. B. (1967). Orientation and order of loci of the met-arg region in the Salmonella typhimurium linkage map. Genetics 56, 463–466.CrossRefGoogle ScholarPubMed
Balish, E. & Shapiro, S. K. (1967). Methionine biosynthesis in Escherichia coli: induction and repression of methylmethionine (or adenosyl methionine): homocysteine methyl-transferase. Arch. Biochem. Biophys. 119, 62–68.CrossRefGoogle Scholar
Demerec, M., Adelberg, E. A., Clark, A. J. & Hartman, P. E. (1966). A proposal for a uniform nomenclature in bacterial genetics. Genetics 54, 61–76.CrossRefGoogle ScholarPubMed
Foster, M. A., Rowbury, R. J. & Woods, D. D. (1963). Control of the methylation of homocysteine in Escherichia coli. J. gen. Microbiol. 31, xix.Google Scholar
Glatzer, L., Labrie, D. A. & Armstrong, F. B. (1966). Transduction of Salmonella typhi-murium–Salmonella montevideo hybrids. Genetics 54, 423–432.CrossRefGoogle ScholarPubMed
Glover, S. W. (1958). A genetical and biochemical study of methionine mutants of Salmonella typhimurium. Proc. VIIth int. Con. Microbiol. 41, 57.Google Scholar
Gross, J. & Englesberg, E. (1959). Determination of the order of mutational sites governing L-arabinose utilization in Escherichia coli B/r by transduction with phage Plbt. Virology 9, 314–331.CrossRefGoogle ScholarPubMed
Jacob, F. & Monod, J. (1961). Genetic regulatory mechanisms in the synthesis of proteins. J. molec. Biol. 3, 318–356.CrossRefGoogle ScholarPubMed
Lawrence, D. A., Smith, D. A. & Rowbury, R. J. (1968). Regulation of methionine synthesis in Salmonella typhimurium: mutants resistant to inhibition by analogues of methionine. Genetics 58, 473–492.CrossRefGoogle ScholarPubMed
Maccacaro, G. A. & Hayes, W. (1961). Pairing interaction as a basis for negative interference. Genet. Res. 2, 406–413.CrossRefGoogle Scholar
Rowbury, R. J. & Woods, D. D. (1966). The regulation of cystathionine formation in Escherichia coli. J. gen. Microbiol. 42, 155–163.CrossRefGoogle ScholarPubMed
Sanderson, K. E. (1967). Revised linkage map of Salmonella typhimurium. Bact. Rev. 31, 354–372.CrossRefGoogle ScholarPubMed
Sanderson, K. E. & Demerec, M. (1965). The linkage map of Salmonella typhimurium. Genetics 51, 897–913.CrossRefGoogle ScholarPubMed
Schlesinger, S. (1967). Inhibition of growth of Escherichia coli and homoserine-O-trans-succinylase by α-methyl-methionine. J. Bact. 94, 327–332.CrossRefGoogle Scholar
Smith, D. A. (1961). Some aspects of the genetics of methionineless mutants of Salmonella typhimurium. J. gen. Microbiol. 24, 335–353.CrossRefGoogle Scholar
Smith, D. A. & Childs, J. D. (1966). Methionine genes and enzymes of Salmonella typhimurium. Heredity 21, 265–286.CrossRefGoogle ScholarPubMed
Taylor, A. L. & Trotter, C. D. (1967). Revised linkage map of Escherichia coli. Bact. Rev. 31, 332–353.CrossRefGoogle ScholarPubMed