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Unstable drug resistance in Staphylococcus aureus M4

Published online by Cambridge University Press:  14 April 2009

W. B. Grubb  and
D. I. Annear
W. B. Grubb
Affiliation:
Biological Laboratories, University of Kent, Canterbury, England, Department of Microbiology, University of Western Australia
D. I. Annear
Affiliation:
Microbiology Department, Royal Perth Hospital, Perth, Western Australia

Summary

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Staphylococcus aureus M4 has chromosomal resistance to streptomycin, plasmid-borne resistance to penicillin and tetracycline and probably chromosomal inducible erythromycin resistance. It also has constitutive erythromycin resistance which is unstable and linked to kanamycin and lincomycin resistance and the ability to produce a diffusible pigment. Variants have been isolated which have stable kanamycin, lincomycin and constitutive erythromycin resistance and these do not produce the diffusible pigment. In transduction experiments kanamycin, lincomycin and constitutive erythromycin resistance were always co-transduced together with streptomycin resistance. The transduction frequencies were approximately 100 times higher with the stable variants compared with the parent. The transductants, irrespective of the donor used, all had stable resistance and did not produce the diffusible pigment. Although transduction with UV-irradiated transducing lysates was characteristic of a plasmid, no corresponding plasmid DNA has been detected.

Type
Research Article
Information
Genetics Research , Volume 38 , Issue 3 , December 1981 , pp. 217 - 223
Copyright
Copyright © Cambridge University Press 1981

References

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