Hostname: page-component-848d4c4894-nmvwc Total loading time: 0 Render date: 2024-07-07T21:36:03.247Z Has data issue: false hasContentIssue false
  • English
  • Français

Dissemination of Bacillus cereus in an Intensive Care Unit

Published online by Cambridge University Press:  21 June 2016

E.A. Bryce ,
J.A. Smith ,
M. Tweeddale ,
B.J. Andruschak  and
M.R. Maxwell
E.A. Bryce*
Affiliation:
Division of Medical Microbiology, Vancouver General Hospital, Vancouver, British Columbia, Canada
J.A. Smith
Affiliation:
Division of Medical Microbiology, Vancouver General Hospital, Vancouver, British Columbia, Canada
M. Tweeddale
Affiliation:
Intensive Care Unit, Department of Medicine, Vancouver General Hospital, Vancouver, British Columbia, Canada
B.J. Andruschak
Affiliation:
Department of Respiratory Services, Vancouver General Hospital, Vancouver, British Columbia, Canada
M.R. Maxwell
Affiliation:
Department of Respiratory Services, Vancouver General Hospital, Vancouver, British Columbia, Canada
*
Division of Medical Microbiology, Vancouver General Hospital, 855 W. 12th Ave., Vancouver, British Columbia., Canada V5Z 1M9
Get access Rights & Permissions [Opens in a new window]

Abstract

Objective:

To report the contamination of ventilator equipment with Bacillus cereus and to outline the measures taken to trace the source of the organism.

Design:

A prospective survey of all intensive care unit patients who were culture-positive for B cereus and obtaining of environmental cultures of the cleaning and assembly area of the respiratory services division between October 1991 and September 1992.

Setting And Patients:

Ventilated patients from a 16-bed medical and surgical intensive care unit (ICU) in a 1,000-bed adult tertiary care hospital.

Interventions And Results:

From October 1991 to April 1992, B cereus colonized the ventilator circuitry of patients in the ICU. One of two washer/decontaminators in the cleaning and assembly area of the respiratory services division was found to yield the microorganism consistently from the water intake port. The design of the machine precluded easy decontamination of the port with 2% gluteraldehyde and a second outbreak occurred. Following the second outbreak, aqueous chlorhexidine in a final concentration of 0.05% was added to the first of two pasteurization cycles in an attempt to achieve sporicidal activity. This ended the outbreak. Sixty-two patients became colonized with the organism including two with nonfatal Bacillus sepsis and one death due to pneumonia associated with the organism.

Conclusion:

This experience emphasizes the importance of obtaining cultures of machine parts to identify the source of contamination and thereby direct control measures. Use of chlor-hexidine gluconate at high temperatures effectively eradicated B cereus from ventilator circuitry in a practical and cost-effective manner.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 14 , Issue 8 , August 1993 , pp. 459 - 462
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Goldstein, B, Abrutyn, E. Pseudo-outbreak of Bacillus species: related to fiberoptic bronchoscopy. J Hosp Infect 1985;6:194–200.CrossRefGoogle Scholar
2. Richardson, AJ, Rothburn, MM, Roberts, C. Pseudo-outbreak of Bacillus species: related to fiberoptic bronchoscopy. J Hasp Infect 1986;7:208–210. Letter.CrossRefGoogle Scholar
3. Weber, DJ, Rutala, WA. Bacillus species. Infect Control Hosp Epidemiol 1988;9:368–373.CrossRefGoogle ScholarPubMed
4. Turnbull, PCB, Kramer, JM. Bacillus. In: Balows, A, Hausler, WJ, Herrmann, KL, Isenberg, LTD, Shadony, LU, eds. Manual of Clinical Microbiology. 5th ed. Washington, DC: American Society for Microbiology; 1991:296–303.Google Scholar
5. LeHau, LA, Benjamin, D, Cantrell, HE Potts, DW, Boggs, JM. Bacillus species pseudomeningitis. Infect Control Hosp Epidemiol 1989;9:394–397.Google Scholar
6. Youngs, ER, Roberts, C, Kramer, JM, Gilbert, RJ. Dissemination of Bacillus cereus in a maternity unit. J Infect 1985;10:228–232.CrossRefGoogle Scholar
7. Richard, V, Van der Auwera, P, Snoeck, R, Daneau, D, Meunier, F. Nosocomial bacteraemia caused by Bacillus species. Eur J Clin Microbiol Infect Dis 1988;7:783–785.CrossRefGoogle ScholarPubMed
8. Gormon, SP, Jones, DS, Loftus, AM. The sporocidal activity and inactivation of chlorhexidine gluconate in aqueous and alcoholic solution. J Appl Bacteriol 1987:63:183–188.CrossRefGoogle Scholar
9. Russell, AD. ‘Bacterial spores and chemical sporicidal agents. Clin Microbiol Rev 1990;3:99–119.CrossRefGoogle ScholarPubMed
10. Shaker, LA, Russell, AD, Furr, JR. Aspects of the action of chlorhexidine on bacterial spores. International Journal of Pharmaceutics 1986;34:51–56.CrossRefGoogle Scholar