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Plasmid Analysis of 26 Staphylococcal Species by a Rapid Microscale Technique

Published online by Cambridge University Press:  02 January 2015

Thomas Herchline  and
William Maher
Thomas Herchline*
Affiliation:
Division of Infectious Disease, Department of Internal Medicine, the Ohio State University College of Medicine, Columbus, Ohio
William Maher
Affiliation:
Division of Infectious Disease, Department of Internal Medicine, the Ohio State University College of Medicine, Columbus, Ohio
*
4881 Sugar Maple Dr, Wright-Patterson AFB, OH 45433
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Abstract

Objective:

Evaluate a plasmid typing technique for a diverse group of staphylococci.

Design:

In vitro testing on known isolates.

Setting:

University hospital.

Intervention:

The plasmid content of 195 isolates representing 26 staphylococcal species was analyzed by agarose gel electrophoresis following lysostaphin-alkaline-SDS lysis, with and without acetone treatment.

Results:

Isolates yielded plasmid profiles with 0 to 7 extra-chromosomal bands (median, 1; 1.5); 171 (88%) had a profile with at least 1 band. Species with more than one isolate available for testing showed considerable diversity of plasmid profiles, except for Staphylococcus haemolyticus.

Conclusions:

The ease of the procedure and the diversity of plasmid profiles within each species examined suggests that plasmid profiling is an accessible and useful epidemiologic tool applicable to most staphylococcal species by epidemiologic units or clinical laboratories.

Type
Molecular Hospital Epidemiology
Information
Infection Control & Hospital Epidemiology , Volume 17 , Issue 10 , October 1996 , pp. 687 - 690
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1996 

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