Hostname: page-component-84b7d79bbc-rnpqb Total loading time: 0 Render date: 2024-08-05T07:08:55.178Z Has data issue: false hasContentIssue false

Pseudo-outbreak of Tuberculosis in an Acute-Care General Hospital: Epidemiology and Clinical Implications

Published online by Cambridge University Press:  02 January 2015

Abstract

A 10-fold increase in patients with Mycobacterium tuberculosis-positive specimens in one hospital laboratory prompted an investigation. Clinical and epidemiological data, along with M tuberculosis DNA fingerprinting results, indicated that laboratory contamination led to nine falsepositive M tuberculosis cultures. Pseudo-infection should be considered in patients with unusual tuberculosis presentations, negative acid-fast bacilli smears, and only one positive culture with a low colony count

Type
Concise Communications
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1998

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

1. van Embden, JDA, Cave, MD, Crawford, JT, Dale, JW, Eisenach, KD, Gicquel, B, et al. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol 1993;31:406409.Google Scholar
2. Michelle, TM, Cronin, WA, Graham, NM, Dwyer, DM, Pope, DS, Harrington, S, et al. Transmission of Mycobacterium tuberculosis by a fiberoptic bronchoscope: identification by DNA fingerprinting. JAMA 1997;278:10931095.Google Scholar
3. Agerton, T, Valway, S, Gore, B, Pozsik, P, Plikaytis, B, Woodley, C, et al. Transmission of a highly drug-resistant strain (strain W1) of Mycobacterium tuberculosis: community outbreak and nosocomial transmission via a contaminated bronchoscope. JAMA 1997;278:10731077.Google Scholar
4. Goodman, RA, Smith, JD, Kubica, GP, Dougherty, EM. Nosocomial mycobacterial pseudoinfection in a Georgia hospital. Infect Control 1984;5:573576.Google Scholar
5. Maurer, JR, Desmond, EP, Lesser, MD, Jones, WD Jr. False-positive cultures of Mycobacteriumi tuberculosis . Chest 1984;86:439443.CrossRefGoogle ScholarPubMed
6. Wurtz, R, Demarais, P, Trainor, W, McAuley, J, Kocka, F, Mosher, L, et al. Specimen contamination of mycobacteria detected by pseudo-outbreak of multi-drug resistant tuberculosis: analysis by routine epidemiology and confirmation by molecular technique. J Clin Microbiol 1997;34:10171019.Google Scholar
7. Braden, CR, Templeton, GL, Stead, WW, Bates, JH, Cave, D, Valway, SE. Retrospective detection of laboratory cross-contamination of Mycobacterium tuberculosis cultures with use of DNA fingerprinting analysis. Clin Infect Dis 1997;24:3540.Google Scholar
8. Dunlap, NE, Harris, RH, Benjamin, WH, Hardin, JW, Hafner, D. Laboratory contamination of Mycobacterium tuberculosis cultures. Am J Respir Crit Care Med 1995;152:17021704.Google Scholar
9. Small, PM, McClenny, NB, Singh, SP, Schoolnik, GK, Tompkins, LS, Mickelsen, PA. Molecular strain typing of Mycobacterium tuberculosis to confirm cross-contamination in the mycobacteriology laboratory and modification of procedures to minimize occurrence of false-positives. J Clin Microbiol 1993;31:1677–682.Google Scholar
10. MacGregor, RR, Clark, LW, Bass, F. The significance of isolating low numbers of Mycobacterium tuberculosis in culture of sputum specimens. Chest 1975;86:518523.Google Scholar