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Use of Nested Polymerase Chain Reaction Based on Sequence-Based Typing of Clinical Samples to Determine the Source of Infection for Hospital-Acquired Legionnaires' Disease

Published online by Cambridge University Press:  02 January 2015

Maria Scaturro ,
Stefano Fontana  and
Maria Luisa Ricci
Maria Scaturro*
Affiliation:
Department of Infectious, Parasitic, and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy
Stefano Fontana
Affiliation:
Department of Infectious, Parasitic, and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy
Maria Luisa Ricci
Affiliation:
Department of Infectious, Parasitic, and Immune-Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy
*
Department of Infectious, Parasitic, and Immune-Mediated Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy (e-mail: maria.scaturro@iss.it)
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Extract

The source of infection of a hospital-acquired Legionnaires' disease case was determined for the first time by nested polymerase chain reaction based on sequence-based typing. The typing was performed directly on DNA extracted from tissue samples, allowing a rapid epidemiological correlation with environmental isolates.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 32 , Issue 5 , May 2011 , pp. 510 - 512
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2011

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