Hostname: page-component-848d4c4894-pjpqr Total loading time: 0 Render date: 2024-06-29T10:12:45.120Z Has data issue: false hasContentIssue false
  • English
  • Français

Evaluation of Real-Time Polymerase Chain Reaction for the Detection of Methicillin-Resistant Staphylococcus aureus on Environmental Surfaces

Published online by Cambridge University Press:  02 January 2015

Jonathan A. Otter ,
Nancy L. Havill  and
John M. Boyce
Jonathan A. Otter*
Affiliation:
Bioquell (UK), Andover, Hampshire, United Kingdom
Nancy L. Havill
Affiliation:
Hospital of Saint Raphael, New Haven, Connecticut
John M. Boyce
Affiliation:
Hospital of Saint Raphael, New Haven, Connecticut Yale University School of Medicine, New Haven, Connecticut
*
Bioquell (UK) Ltd., 34 Walworth Road, Andover, Hampshire, SP10 5AA, UK (jon.otter@bioquell.com)
Get access Rights & Permissions [Opens in a new window]

Abstract

We compared real-time polymerase chain reaction (RT-PCR) with in vitro culture for detecting methicillin-resistant Staphylococcus aureus in samples from environmental surfaces. The sensitivity of RT-PCR, compared with culture, was 92.5%, and the specificity was 51.4%. Because of poor specificity, the RT-PCR kit tested is not suitable for the detection of MRSA on hospital surfaces.

Type
Concise Communications
Information
Infection Control & Hospital Epidemiology , Volume 28 , Issue 8 , August 2007 , pp. 1003 - 1005
Copyright
Copyright © The Society for Healthcare Epidemiology of America 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1. Muto, CA, Jernigan, JA, Ostrowsky, BE, et al. SHEA guideline for preventing nosocomial transmission of multidrug-resistant strains of Staphylococcus aureus and Enterococcus . Infect Control Hosp Epidemiol 2003;24:362–386.CrossRefGoogle ScholarPubMed
2. Coia, JE, Duckworth, GJ, Edwards, DI, et al. Guidelines for the control and prevention of methicillin-resistant Staphylococcus aureus (MRSA) in healthcare facilities. J Hosp Infect 2006;63:1–44.CrossRefGoogle ScholarPubMed
3. Warren, DK, Liao, RS, Merz, LR, Eveland, M, Dunne, WM Jr. Detection of methicillin-resistant Staphylococcus aureus directly from nasal swab specimens by a real-time PCR assay. J Clin Microbio? 2004;42:5578–5581.CrossRefGoogle ScholarPubMed
4. Huletsky, A, Giroux, R, Rossbach, V, et al. New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci. J Clin Microbiol 2004;42:1875–1884.CrossRefGoogle ScholarPubMed
5. Boyce, JM, Potter-Bynoe, G, Chenevert, C, King, T. Environmental contamination due to methicillin-resistant Staphylococcus aureus: possible infection control implications. Infect Control Hosp Epidemiol 1997;18:622–627.CrossRefGoogle ScholarPubMed
6. French, GL, Otter, JA, Shannon, KP, Adams, NM, Watling, D, Parks, MJ. Tackling contamination of the hospital environment by methicillin-resistant Staphylococcus aureus (MRSA): a comparison between conventional terminal cleaning and hydrogen peroxide vapour decontamination. J Hosp Infect 2004;57:31–37.CrossRefGoogle ScholarPubMed
7. Hardy, KJ, Oppenheim, BA, Gossain, S, Gao, F, Hawkey, PM. A study of the relationship between environmental contamination with methicillin-resistant Staphylococcus aureus (MRSA) and patients' acquisition of MRSA. Infect Control Hosp Epidemiol 2006;27:127–132.CrossRefGoogle ScholarPubMed
8. Artz, RR, Avery, LM, Jones, DL, Killham, K. Potential pitfalls in the quantitative molecular detection of Escherichia coli O157:H7 in environmental matrices. Can J Microbiol 2006;52:482–488.CrossRefGoogle ScholarPubMed
9. Desjardins, M, Guibord, C, Lalonde, B, Toye, K, Ramotar, B. Evaluation of the IDI-MRSA assay for detection of methicillin-resistant Staphylococcus aureus from nasal and rectal specimens pooled in a selective broth. I Clin Microbiol 2006;44:1219–1223.CrossRefGoogle Scholar
10. Flayhart, D, Hindler, JF, Bruckner, DA, et al. Multicenter evaluation of BBL CHROMagar MRSA Medium for direct detection of methicillin-resistant Staphylococcus aureus from surveillance cultures of the anterior nares. J Clin Microbiol 2005;43:5536–5540.CrossRefGoogle ScholarPubMed