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Evaluation of the Variety of Plasmid Profiles in S epidermidis Isolates from Hospital Patients and Staff

Published online by Cambridge University Press:  21 June 2016

Carrie R. Valentine ,
Susan H. Yandle ,
Frederic J. Marsik ,
Jack R. Ebright  and
Mark S. Dawson
Carrie R. Valentine*
Affiliation:
Departments of Microbiology/Immunology and Internal Medicine, Oral Roberts University and City of Faith, Tulsa, Oklahoma
Susan H. Yandle
Affiliation:
Departments of Microbiology/Immunology and Internal Medicine, Oral Roberts University and City of Faith, Tulsa, Oklahoma
Frederic J. Marsik
Affiliation:
Departments of Microbiology/Immunology and Internal Medicine, Oral Roberts University and City of Faith, Tulsa, Oklahoma
Jack R. Ebright
Affiliation:
Departments of Microbiology/Immunology and Internal Medicine, Oral Roberts University and City of Faith, Tulsa, Oklahoma
Mark S. Dawson
Affiliation:
Departments of Microbiology/Immunology and Internal Medicine, Oral Roberts University and City of Faith, Tulsa, Oklahoma
*
Department of Microbiology/Immunology, Oral Roberts University, Tulsa, OK 74137-1297
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Abstract

Plasmid profiling was used to determine the variability of normal flora isolates of Staphylococcus epidermidis in order to evaluate the usefulness of plasmid profiling for identifying pathogens. Fifteen hospital staff members and patients repeatedly had cultures taken from the hands and nares, and multiple isolates were examined for plasmid profiles. S epidermidis isolated from the nares of 15 neonates were also examined. The total number of isolates examined for plasmid profiles was 726. Repetition of profiles was common among the different isolates from a single sampling (one swab). The frequency of re-isolating similar profiles on different days varied from 7% to 13%. Simultaneous isolation of similar profiles from nares and hands on the same individual varied from 0% to 11%, the percentage being lower for personnel. Isolation of the same plasmid profile from different individuals occurred only twice and resulted in an assignment probability of Pa = 0.002 for isolates obtained from different individuals. Significantly more isolates from nares contained plasmids (97%) compared with isolates from hands (89%).Patients who had two or more isolates of coagulase-negative staphylococci with similar profiles were judged, clinically, to have infections in 12 of 13 cases. However, the likelihood of re-isolating an S epidermidis strain with a similar plasmid profile twice from the same person at different times was sufficiently high to prevent plasmid profiling from being used as an absolute criterion for infection.

Type
Original Articles
Information
Infection Control & Hospital Epidemiology , Volume 9 , Issue 10 , October 1988 , pp. 441 - 446
Copyright
Copyright © The Society for Healthcare Epidemiology of America 1988

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