Background: Effective hand hygiene (HH) is an essential preventative measure for the reduction of hospital-acquired infections (HAIs). Commonly used HH products include alcohol-based hand rubs (ABHRs), antimicrobial soaps, and nonantimicrobial soaps. In vivo clinical studies have demonstrated that levels of bacterial reduction can vary based on the HH product type, formulation, and dose. It has been reported that ABHRs provide the greatest reduction in bacteria, followed by antimicrobial soaps. Objective: We examined the effects of products representative of 3 HH categories on artificially soiled hands, using a hand-stamp procedure. The hand-stamp images provide a clear visualization of product effectiveness and can be used as an educational tool to promote the importance of proper hand hygiene using different product formats. Method: Three commercially available formulations were evaluated in this study, a mild nonantimicrobial soap, an antimicrobial soap containing chloroxylenol (PCMX), and an ABHR containing 70% v/v ethanol. Prior to the hand stamp procedure, the participant’s hands were prewashed with 5 mL of a nonantimicrobial soap and dried. An inoculum of Serratia marcescens containing ∼1 × 109CFU/mL was prepared as described in ASTM E2755. A 0.2-mL aliquot of the inoculum was dispensed onto the palm of the subject’s hand and spread by rubbing over the entire surface of both hands. Following a 30-second dry time, one of the subject’s hands was gently pressed onto the surface of a large petri dish containing tryptic soy agar to obtain a baseline image. Following the baseline sample, 1 pump of the selected test product (∼0.9 mL for soap or 1.1 mL for ABHR) was applied to the participant’s hands. For soap applications, hands were vigorously rubbed for 30 seconds followed by a 30-second water rinse. For ABHR, product was rubbed by the user until dry. The hand-stamp procedure was repeated following product application using the participant’s other hand. Results: Clear qualitative reductions in bacteria were observed with each of the HH interventions. The greatest reduction was observed following the application of ABHR. Antimicrobial soap was less effective than ABHR but more effective than nonantimicrobial soap. Conclusions: The qualitative visual model demonstrates the effectiveness of various HH interventions and correlates with log reductions observed in traditional efficacy test methods. Future efforts should explore hand-stamp repeatability and image utilization to support HH improvement efforts in healthcare systems.

Funding: GOJO Industries provided support for this study.

Disclosures: Mary Rose Czaplicki reports salary from GOJO Industries.