The very first spectrograms of 40 Seyfert galaxies are discussed. All these objects were taken from Markarian’s lists of galaxies. They were first investigated and classified at Fesenkov Astrophysical Institute during 1973-1988. Spectral study was carried out with a slit spectrograph, attached to the 70-cm telescope and equipped with a three cascades image-tube. Repeated observations of 22 galaxies were obtained in 1985-2000. An analysis of possible spectral variability of the studied galaxies over 10-26 years was made. A list of objects which are the most interesting for further observations is presented.