Hostname: page-component-77c89778f8-cnmwb Total loading time: 0 Render date: 2024-07-19T11:59:09.388Z Has data issue: false hasContentIssue false
  • English
  • Français

Dose transfer of an oil-based formulation of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) sprays to cotton bollworm in an arena trial

Published online by Cambridge University Press:  13 January 2012

O.K. Douro Kpindou ,
D.A. Djegui ,
I.A. Glitho  and
M. Tamò
O.K. Douro Kpindou*
Affiliation:
International Institute of Tropical Agriculture, 08 BP 0932, Tri Postal, Cotonou, Benin
D.A. Djegui
Affiliation:
International Institute of Tropical Agriculture, 08 BP 0932, Tri Postal, Cotonou, Benin
I.A. Glitho
Affiliation:
Laboratoire d'Entomologie Appliquée, Faculté des Sciences, Université de Lomé, BP 1515, Lomé, Togo
M. Tamò
Affiliation:
International Institute of Tropical Agriculture, 08 BP 0932, Tri Postal, Cotonou, Benin
*
*E-mail: d.kpindou@cgiar.org
Get access

Abstract

A cotton field was used to set up an arena trial for investigating the dose transfer of oil-based ultra-low-volume sprays of Metarhizium anisopliae (Metchnikoff) Sorokin to the cotton bollworm Helicoverpa armigera (Hübner). Two doses of conidia (75 and 50 g/ha, being 3.75 × 1012 and 2.5 × 1012 conidia/ha, respectively) formulated in 2 litres (70:30, kerosene–peanut oil) were applied using a Berthoud ‘Micro Ulva’ to three groups of larvae of cotton bollworms positioned in three rows downwind of a single spray line. Both the distribution of the droplets and the effect of direct contact with spray droplets were quantified. The mean number of droplets decreased from 416 ( ± 60) droplets per cm2 (line 1; 0.4 m) to 45 ( ± 36) droplets per cm2 (line 3; 5 m). To achieve 50% mortality, >10 and >13 days were necessary for 75 and 50 g/ha, respectively. From day 15, the difference in mortality rates was significant between the two treatments. At line 3 (5 m), at 12 days after application, the mortality rate reached 52% for 75 g/ha and 47% for 50 g/ha. The results are discussed in view of optimizing the application of the biopesticides.

Keywords

Helicoverpa armigeraMetarhizium anisopliaebiopesticidedose transfercotton fieldultra-low-volume application
Type
Research Paper
Information
International Journal of Tropical Insect Science , Volume 31 , Issue 4 , December 2011 , pp. 262 - 268
Copyright
Copyright © ICIPE 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bateman, R. P. (1993) Simple, standardised methods for recording droplet measurements and estimation of deposits from controlled droplet applications. Crop Protection 12, 201–206.CrossRefGoogle Scholar
Bateman, R. P. (1997) Methods of application of microbial pesticide formulations for the control of locusts and grasshoppers. Memoirs of the Entomological Society of Canada 171, 69–81.CrossRefGoogle Scholar
Bateman, R. P., Douro-Kpindu, O. K., Kooyman, C., Lomer, C. and Ouambama, Z. (1998) Some observations on the dose transfer of mycoinsecticide sprays to desert locusts. Crop Protection 17, 151–158.CrossRefGoogle Scholar
Bateman, R. P., Godonou, I., Douro Kpindou, O. K., Lomer, C. J. and Paraiso, A. (1992) Development of a novel “field bioassay” technique for assessing mycopesticide U.L.V. formulations, pp. 255–262. In Biological Control of Locusts and Grasshoppers (edited by Lomer, C. J. and Prior, C.). CAB International, Wallingford.Google Scholar
Bateman, R. P., Price, R. F., Müller, E. J. and Brown, H. D. (1994) Controlling brown locust hopper bands in South Africa with a myco-insecticide spray, pp. 609–616. Proceedings of Brighton Crop Protection Conference – Pest and Diseases, 16–19 November 1994, Brighton, UK.Google Scholar
Bateman, R. P. and Thomas, M. (1996) Pathogen application against locusts and grasshoppers: insecticide or biological control? Antenna 20, 10–15.Google Scholar
Cherry, A., Jenkins, N., Heviefo, G., Bateman, R. P. and Lomer, C. (1999) A West African pilot scale production plant for aerial conidia of Metarhizium sp. for use as a mycoinsecticide against locusts and grasshoppers. Biocontrol Science and Technology 9, 35–51.CrossRefGoogle Scholar
Douro Kpindou, O. K., Djegui, D. A., Glitho, I. A. and Tamò, M. (2010) Réponse des stades larvaires de Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) à différentes doses d'application des champignons entomopathogènes Metarhizium anisopliae et Beauveria bassiana. Biotechnologie Agronomie et Environnement.Google Scholar
Douro-Kpindou, O.-K., Godonou, I., Houssou, A., Lomer, C. J. and Shah, P. A. (1995) Control of Zonocerus variegatus with ULV formulation of Metarhizium flavoviride conidia. Biocontrol Science and Technology 5, 131–139.CrossRefGoogle Scholar
Hewitt, H. G., Caseley, J., Copping, L. G., Grayson, B. T. and Tyson, D. (eds) (1994) Comparing glasshouse and field pesticide performance 11, British Crop Protection Council Monograph No. 59. 323 pp.Google Scholar
Kulkarni, S. A., Ghormade, V., Kulkarni, G., Kapoor, M., Chavan, S. B., Rajendran, A., Patil, S. K., Shouche, Y. and Deshpande, M. V. (2008) Comparison of Metarhizium isolates for biocontrol of Helicoverpa armigera (Lepidoptera: Noctuidae) in chickpea. Biocontrol Science and Technology 18, 809–828.CrossRefGoogle Scholar
Kumar, V. and Chowdhry, P. N. (2004) Virulence of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against tomato fruit borer, Helicoverpa armigera. Indian Phytopathological Society 54, 208–212.Google Scholar
Langewald, J., Thomas, M. B., Douro Kpindou, O. K. and Lomer, C. J. (1997) Use of Metarhizium flavoviride for control of Zonocerus variegatus: a model, linking dispersal and secondary infection from the spray residue with mortality in caged field samples. Entomologia Experimentalis et Applicata 82, 1–8.CrossRefGoogle Scholar
Lomer, C. J., Bateman, R. P., Godonou, I., Douro Kpindou, O. K., Shah, P. A., Paraiso, A. and Prior, C. (1993) Field infection of Zonocerus variegatus following application of an oil-based formulation of Metarhizium flavoviride conidia. Biocontrol Science and Technology 3, 337–346.CrossRefGoogle Scholar
Matthews, G. A. (1992) Pesticide Application Methods, 2nd edn.Longmans, Harlow. 405 pp.Google Scholar
SAS (2002–2008). SAS® 9.2, Copyright © 2002–2008 by SAS Institute Inc., Cary, NC, USA.Google Scholar
Staniland, L. N. (1959) Fluorescent tracer for the study of spray and dust deposits. Journal of Agricultural Engineering Research 4, 42–81.Google Scholar
Symmons, P. M., Dobson, H. M. and Sissoko, M. (1991) Pesticide droplet size and efficacy: a series of trials on grasshoppers. Crop Protection 10, 136–144.CrossRefGoogle Scholar
Teakle, R. E. and Jensen, J. M. (1985) Heliothis punctigera, pp. 313–322. In Handbook of Insect Rearing (edited by Singh, P. and Moore, R. F.). Elsevier, Amsterdam.Google Scholar
Thomas, M. B., Wood, S. N. and Lomer, C. J. (1995) Biological control of locusts and grasshoppers using a fungal pathogen: the importance of secondary cycling. Proceedings of the Royal Society London (B) 259, 265–270.Google Scholar