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Effect of Cyanocobalamin on Protein and Nucleic Acid Contents of Ovary of Silkworm, Bombyx mori L., During Larval, Pupal and Adult Stages of Development

Published online by Cambridge University Press:  19 September 2011

Subrata Das  and
A. K. Medda
Subrata Das
Affiliation:
Department of Animal Physiology, Bose Institute, P-1/12 C.I.T. Scheme VII M, Kankurgachi, Calcutta-700054, India
A. K. Medda
Affiliation:
Department of Animal Physiology, Bose Institute, P-1/12 C.I.T. Scheme VII M, Kankurgachi, Calcutta-700054, India
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Abstract

The effects of cyanocobalamin on the protein, RNA and DNA contents of ovary of silkworms, Bombyx mori L., were investigated. Two sets of experiments were performed, one set dealing with the changes on some selected days in protein, RNA and DNA contents after a single injection of different doses of cyanocobalamin (0.25, 0.5, 1, 2 and 5 μg/g of body weight), given on 2nd day of fifth larval instar (day 0), and the other dealing with the day-to-day variations of these cellular constituents and the effect of a particular dose (2 μg/g) of cyanocobalamin on the daily changes of the substances. In the first set of experiments, the enhanced protein content of ovary over the control value was found on day 8 (2nd day of pupa) and day 13 (adult stage) with 1, 2 and 5 μg of cyanocobalamin/g. Cyanocobalamin at the dose of 0.25 and 0.5 μg/g was ineffective in causing any change in protein content. Increased RNA content was noted in comparison to the control on days 3 (5th day of fifth instar), 8 and 13. In case of DNA content of ovary, the enhancement was observed on days 8 and 13 with 1, 2 and 5 μg/g doses. It was further evident from day-to-day changes that protein, RNA and DNA in ovary followed a specific individual pattern of changes during the developmental period under study. Cyanocobalamin at the dose of 2 μg/g increased protein content of ovary from day 5 to day 13, RNA content from day 3 to day 13 and DNA content from day 4 to day 13 without altering the specific pattern of variation of these cellular constituents. It was further observed that ovarian weight and egg number increased with different doses (0.5–5 μg/g) of the vitamin.

Résumé

Des recherches ont été faites sur les effets causés par le cyanocobalamin sur les contenus en protéïnes, RNA et DNA du ver à soie, Bombyx mori L. On mené à bien deux séries d'expériences. L'une traite des changements notés sur certains jours choisis, en ce qui concerne les contenus en protéïnes, RNA et DNA après une injection unique de doses différentes de cyanocobalamin (0,25, 0,5, 1, 2 et 5 μg/gramme de poids de corps), donnée le deuxième jour après le 5ème stade larval (jour 0). L'autre traite des variations quotidiennes de ces composants cellulaires et de l'effet d'une dose particulière (2 μg/g) de cyanocobalamin sur les changements quotidiens des substances. Dans la première série d'expériences l'augmentation du contenu en protéïnes dans les ovairespar rapport à la valeur de contrôle fut remarquée au jour 8 (2 ème jour de pupa) et au jous 13 (stage adulte) avec 1, 2 et 5 μg/g de cyanocobalamin/g. A une dose de 0,25 et 0,5 μg/g, le cyanocobalamin n'a pas altéré le contenu en protéines. On a remarqué une augmentation du RNA en comparaison avec le contrôle aux jours 3 (5ème jour du 5ème stade larval), 8 et 13. En cas de contenu de DNA dans les ovaires, l'augmentation a été observée aux jours 8 et 13, avec des doses de 1, 2 et 5 μg/g. Il a ensuite semblé évident que, selon les changements quotidiens, les protéïnes, le RNA et le DNA dans les ovaires suivaient un mode individuel bien spécifique de modifications au cours de la péode de développement qui est étudiée. Une dose de 2 μg/g de cyanocobalamin augmentait le contenu en protéines des ovaires du jour 5 au jour 13, le contenu en RNA du jour 3 au jour 13 et le contune en DNA du jour 4 au jour 13, sans modifier le mode spécifique de variation de ces composants cellulaires. Il a été ensuite observé que la poids des ovaires et le nombre d'oeufs augmentaient avec des doses différentes (0.5–5 μg/g) de la vitamine.

Keywords

CyanocobalaminBombyx moriovaryproteinRNADNACyanocobalaminBombyx moriovairesprotéïnesRNADNA
Type
Research Article
Information
International Journal of Tropical Insect Science , Volume 9 , Issue 5 , October 1988 , pp. 641 - 646
Copyright
Copyright © ICIPE 1988

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