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A Simple and Rapid Method for Mini Preparation of High Molecular Weight DNA from Certain Acarines, Bacteria and Soybean

Published online by Cambridge University Press:  19 September 2011

Z. A. El-Fiky
Z. A. El-Fiky
Affiliation:
Genetics Department, Faculty of Agriculture, Fayoum Branch Cairo University, Fayoum, Egypt
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Abstract

A simple, rapid and low-cost method for the isolation of high molecular weight DNA from small amounts of cells or tissue is described. The method does not require liquid nitrogen, proteinase K or costly buffers. This method was successfully applied in the isolation and purification of DNA from eggs of Hyalomma excavatum, H. dromedarii, Argas persicus, A. hermanii and Boophilus annulatus, in addition to Escherichia coli, actinomycetes (Nocardia sp.) and leaves of soybean (Glycine max L.). The DNA concentration was calculated from the optical density (OD) value at wavelength 260 nm and the ratios OD260/OD280 and OD260/OD280 determined. The quality and amount of DNA obtained by this method were comparable to those of DNA isolated from equal amounts of material using a commercial genomie DNA purification kit.

Résumé

Cette publication présente une méthode simple, rapide et peu coûteuse d'isolement de l'ADN de poids moléculaire élevé à partir d'une petite quantité de cellules ou de tissus. La méthode ne nécessite ni hydrogène liquide, ni protéinase K, ni même de tampons coûteux. Cette méthode a été appliquée avec succès pour l'isolement et la purification d'ADN d'oeufs de Hyalomma excavatum, de H. dromedarii, d'Argas persicus, d'A. hermanii et de Boophilus annulatus, ainsi que d'Escherichia coli, de l'actinomycète (Nocardia sp.) et de feuilles de soja (Glycine max L.). La concentration d'ADN est calculée par la méthode des densités optiques (DO) pour une longueur d'onde de 260 nm et les rapports DO260/DO280etDO260/DO230 sont déterminés. La qualité et la quantité d'ADN obtenues par cette méthode sont comparables à celles isolées de quantités identiques de matériel à partir d'un kit commercial de purification d'ADN génomique.

Keywords

DNA extractionsimple methodHyalomma excavatumH. dromedariiArgas persicusA. hermaniiBoophilus annulatusEscherichia coliNocardia sp.Glycine maxextraction d'ADNméthode simpleHyalomma excavatumH. dromedariiArgas persicusA. hermaniiBoophilus annulatusEscherichia coliNocardia sp.Glycine max
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 23 , Special Issue 1: Novel Approaches to Tick and Mite Management in Africa , March 2003 , pp. 51 - 57
Copyright
Copyright © ICIPE 2003

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References

REFERENCES

Aboytes-Torres, R. (1992) Development and practical applications of a PCR based diagnostic system for the study of chronic bovine anaplasmosis. PhD Dissertation. University of Missouri, Columbia, MO, USA.Google Scholar
Bloom, R.A., Mullin, B.C. and Tate, R.L. (1989) DNA restriction patterns and solution hybridization studies of Frankia isolates from Myrica pensylvanica (bayberry). Appi. Environ. Microbiol. 55, 2155–2160.CrossRefGoogle Scholar
Couch, J.A. and Fritz, P.J. (1990) Isolation of DNA from plants high in polyphenolics. Plant Mol. Biol. Rep. 8, 8–12.CrossRefGoogle Scholar
de Kok, J.B., d'Oliveira, C. and Jongejan, F. (1993) Detection of the protozoan parasite Theileria annidata in hyalomma ticks by the polymerase chain reaction. Exp. Appi. Acarol. 17, 839–846.Google Scholar
Dellaporta, S.L., Wood, J. and Hicks, J.B. (1983) A plant DNA mini preparation. Version III, Plant Mol. Biol. 1, 19–21.Google Scholar
d'Oliveira, C., van der Weide, M., Jacquiet, P. and Jongejan, F. (1997) Detection of Theileria annidata by PCR in ticks collected from cattle in Mauritania. Exp. Appi. Acarol. 21, 279–291.Google Scholar
Gage, K.L., Schrumpf, M.E., Karstens, R.H., Burgdorfer, W. and Schwan, T.G. (1994) DNA typing of rickettsiae in naturally infected ticks using a PCR/RFLP system. Amer. J. Trop. Med. Hyg. 50, 247–260.Google Scholar
Guillemaut, P. and Marechal-Drouard, L. (1992) Isolation of plant DNA: A fast, inexpensive and reliable method. Plant Mol. Biol. Rep. 10, 60–65.CrossRefGoogle Scholar
Guttman, D.S., Wang, P.W., Wang, I.N., Bosler, E.M., Luft, B.J. and Dykhuizen, D.E. (1996) Multiple infections of Ixodes scapularis ticks by Borrelia burgdorferi as revealed by single strand conformation polymorphism analysis, J. Clin. Microbiol. 34, 652–656.CrossRefGoogle ScholarPubMed
Hubbard, M.J., Cann, K.J. and Wright, D.J.M. (1995) Validation and rapid extraction of nucleic acids from alcohol-preserved ticks. Exp. Appi. Acarol. 19, 473–478.Google Scholar
Jhingan, A. K. (1994) DNA isolation from animal cells. PATN Patent Bibliographic Information, Gen Bank No. 05352777.Google Scholar
Jongejan, F., van Vliet, A.H.M., van der Weide, M. and d'Oliveira, C. (1998) Detection of two tick-borne pathogens (Theileria annulata and Cowdria ruminantium)by the polymerase chain reaction, pp. 521–525. In Proceedings of the Second International Conference on Tick-borne Pathogens. Kruger National Park. Republic of South Africa, 28 August-1 September 1995.Google Scholar
Junghans, H. and Metzlaff, M. (1990) A simple and rapid method for the preparation of total plant DNA. Biotechniques 8, 176.Google Scholar
Kamalay, J.C., Tejwani, R. and Rufener, G.K. (1990) Isolation and analysis of genomie DNA from single seeds. Crop Science 30, 1079–1084.Google Scholar
Katterman, F.R.H. and Shattuck, V.I. (1983) An effective method of DNA isolation from the mature leaves of Gossypium species that contain large amounts of phenolic terpenoids and tannins. Preparative Biochemistry 13, 347–359.Google Scholar
Marmur, J. (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms, J. Mol. Biol. 3, 208–218.CrossRefGoogle Scholar
Mauel, M.J., Carlton, S.J. and Mather, T. N. (1999) Polymerase chain reaction detection efficiency of the human granulocytic ehrlichiosis agent (Rickettsiaceae: Ehrlichieae) in ticks (Acari: Ixodidae) is dependent on the DNA extraction method, J. Med. Entomol. 36, 649–652.CrossRefGoogle ScholarPubMed
Peterson, D.G., Boehm, K.S. and Stack, S.M. (1997) Isolation of milligram quantities of nuclear DNA from tomato (Lycopersicon esculentum), a plant containing high levels of polyphenolic compounds. Plant Mol. Biol. Rep. 15, 148–153.Google Scholar
Ramelow, C., Suss, J., Berndt, D., Roggendorf, M. and Schreier, E. (1993) Detection of tick-borne encephalitis virus RNA in tick (Ixodes ricinus) by the PCR. J. Virol. Meth. 45, 115–119.CrossRefGoogle Scholar
Sambrook, J., Fritsh, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edition (Edited by Nolan, C.). Cold Spring Harbor Laboratory Press, USA.Google Scholar
Shatters, R.G., Schweder, M.E., West, S. H., Abdelghany, A. and Smith, R.L. (1995) Environmentally induced polymorphisms detected by RAPD analysis of soybean seed DNA. Seed Sci. Res. 5, 109–116.Google Scholar
Sparagano, O.A.E., Allsopp, M.T.E.P., Mank, R.A., Rijpkema, S.G.T., Figueroa, J.V. and Jongejan, F. (1999) Molecular detection of pathogen DNA in ticks (Acari: Ixodidae): A review. Exp. Appi. Acarol. 12, 929–960.CrossRefGoogle Scholar
Walbot, V. (1989) Preparation of DNA from single rice seedlings. Rice Genetics Newsl. 5, 149–151.Google Scholar
Ward, V.K., Marriott, A.C., Booth, T.F., El-Ghorr, A.A. and Nuttall, P.A. (1990) Detection of an arbovirus in an invertebrate and a vertebrate host using the PCR. J. Virol. Meth. 30, 291–300.CrossRefGoogle Scholar
Watson, J.C. and Thompson, W.F. (1986) Purification am restriction endonuclease analysis of plant nuclea DNA. Methods Enzymol. 118, 57–79.Google Scholar
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. (1990) DNA polymorphism amplified by arbitrary primers are useful as generl. markers. Nucleic Adds Res. 18, 6531–6535.CrossRefGoogle Scholar
Winfrey, M.R., Rott, M.A. and Wortman, A.T. (1997) Unravelling DNA: Molecular Biology for the Laboratory Prentice Hall Inc., Simon & Schuster/A. Viacom Company, New Jersey. 396 pp.Google Scholar