Hostname: page-component-5c6d5d7d68-sv6ng Total loading time: 0 Render date: 2024-08-23T15:29:59.014Z Has data issue: false hasContentIssue false

Chemotaxis of Cicer Rhizobium strains to root exudates of chick pea (Cicer arietinum L.) genotypes and their interaction response on nodulation, nodulins, leghaemoglobin and grain yield in calcareous soil

Published online by Cambridge University Press:  27 March 2009

R. Rai
Affiliation:
Rajendra Agricultural University, Dholi Campus, Dholi, Muzaffarpur 843121, Bihar, India
V. Prasad
Affiliation:
Rajendra Agricultural University, Dholi Campus, Dholi, Muzaffarpur 843121, Bihar, India

Summary

Two Rhizobium strains were studied for their chemotaxis, nodulation, nitrogenase activity and other endosymbiotic N2-fixing characteristics with two genotypes of Cicer arietinum. Root exudates were found to attract Rhizobium strains. Nodule-specific root proteins (nodulins) and leghaemoglobin were found to be host-range specific between two strains of Cicer Rhizobium. Physiologically active iron content was more important for nodule functioning than total iron concentration of nodules.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1986

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Armitage, J. P., Josey, D. P. & Smith, D. G. (1977). A simple, quantitative method for measuring chemotaxis and motality in bacteria. Journal of General Microbiology 102, 199201.Google Scholar
Auger, S. & Verma, D. P. S. (1981). Induction and expression of nodule-specific host genes in effective and ineffective root nodules of soybean. Biochemistry 20, 13001306.CrossRefGoogle ScholarPubMed
Beringer, J. E., Brewin, N., Johnston, A. W. B., Schulman, H. M. & Hopwood, D. A. (1979). The Rhizobium-legume symbiosis. Proceedings of the Royal Society London (B) 204, 219233.Google Scholar
Bisseling, T., Been, C, Klugkist, J., Van Kammen, A. & Nadler, K. (1983). Nodule-specific host proteins in effective and ineffective root nodules of Piaum sativum. The EMBO Journal 2, 961966.CrossRefGoogle ScholarPubMed
Bowra, B. J. & Dilworth, M. J. (1981). Motality and chemotaxis towards sugars in Rhizobium leguminosarum. Journal of General Microbiology 126, 231235.Google Scholar
Currier, W. W. & Stobel, C. A. (1977). The chemotactic behaviour of trefoil Rhizobium. FEMS Microbiology Letters 1, 243246.Google Scholar
Fuller, F., Kunstner, P., Nguyen, T. & Verma, D. P. S. (1983). Nodulin genes of soybean: analysis of CDNA clones reveals several abundant sequences in nitrogen fixing root nodules. Proceedings of National Academy of Sciences U.S.A. 80, 25942598.CrossRefGoogle ScholarPubMed
Gaworzewska, E. T. & Carlile, M. J. (1982). Positive chemotaxis of Rhizobium leguminosarum and other bacteria towards root exudates from legumes and other plants. Journal of General Microbiology 128, 11791188.Google Scholar
Gitte, R. R., Rai, P. V. & Patil, R. B. (1978). Chemotaxis of Rhizobium sp. towards root exudates of Cicer arietinum L. Plant and Soil 50, 553566.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. K. & Randall, R. J. (1951). Protein measurement with Folin phenol reagent. Journal of Biological Chemistry 193, 265275.CrossRefGoogle ScholarPubMed
Rai, P. V. & Strobel, G. A. (1966). Chemotaxis of Zoospores of Aphanomyces cochliodes to sugar beet seedlings. Phytopathology 56, 13651368.Google Scholar
Rai, R., Prasad, V., Choudhury, S. K. & Sinha, N. P. (1984). Iron nutrition and symbiotic N2 fixation of lentil (Lens culinaris) genotypes in calcareous soil. Journal of Plant Nutrition 7 (1–5), 399405.CrossRefGoogle Scholar
Rai, R., Prasad, V., Sharma, S. D. & Shukla, I. C. (1983). Competitive ability, effectiveness and succinate tryphenyl tetrazobium chloride reductase activity of mutant strains of chick pea (Cicer arietinum L.) Rhizobium sp. Canadian Journal of Microbiology 29, 735745.Google Scholar
Rai, R. & Singh, S. N. (1979). Interaction between chick pea (Cicer arietinum Linn.) genotypes and strains of Rhizobium sp. Journal of Agricultural Science, Cambridge 92, 437441.CrossRefGoogle Scholar
Schiffman, J. & Lobel, R. (1970). Haemoglobin determination and its value as an early indication of peanut Rhizobium efficiency. Plant and Soil 33, 501512.Google Scholar
Somogyi, M. (1952). Notes on sugar determination. Journal of Biological Chemistry 195, 1923.Google Scholar
Verma, D. P. S. (1984). Involvement of specific, host genes in symbiotic nitrogen fixation. In Genetics: New Frontiers (ed. Chopra, V. L., Joshi, B. C., Sharma, R. P. and Bansal, H. C.), pp. 227239. New Delhi: Oxford and IBH Publishing Company.Google Scholar
Verma, D. P. S. & Long, S. (1983). The molecular biology of the Rhizobium–legame symbiosis. International Review of Cytology 14, 211245.Google Scholar