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The effect of changes in the intestinal flow of nucleic acids on allantoin excretion in the urine of Sheep

Published online by Cambridge University Press:  27 March 2009

Anna M. Antoniewicz ,
W. W. Heinemann  and
E. M. Hanks
Anna M. Antoniewicz
Affiliation:
Department of Animal Nutrition, Institute of Animal Production, Sarego 2, Krakow, Poland
W. W. Heinemann
Affiliation:
Irrigated Agriculture Research and Extension Center, Washington State University, Prosser, WA 99350, U.S.A.
E. M. Hanks
Affiliation:
Irrigated Agriculture Research and Extension Center, Washington State University, Prosser, WA 99350, U.S.A.
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Summary

Three ewes were intraduodenally infused with yeast RNA (15, 20 and 30 g/day) or control solutions for 3 days and the net changes in urinary allantoin excretion determined. Four mature wethers fitted with a re-entrant cannula in the proximal duodenum were fed two levels (0·66 and 1 × maintenance) of pelleted lucerne hay. Allantoin excretion was compared with total nucleic acid (NA) flow in the small intestine.

The average proportion of the recovery of the infused RNA-N dose as urinary allantoin–N amounted to 0·119 ± 0·0046. The values of the net increase of allantoin-N (Y, g/day) above control levels were highly significantly correlated (n = 12, r = 0·98) with amounts of RNA-N infused (X, g/day): y = 0·1185 (± 0·0086) X–0·004 (P < 0·001). The average ratio of urinary allantoin-N to NA-N passing the duodenum during 24 h was 0·173. A difference between the two conversion factors is discussed in relation to endogenous allantoin excretion and purine base composition of yeast and bacterial RNA.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 95 , Issue 2 , October 1980 , pp. 395 - 400
Copyright
Copyright © Cambridge University Press 1980

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