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Excretion patterns of 3-hydroxy-4(1H) pyridone (DHP) by steers fed a high energy and a high-fibre diet supplemented with Leucaena leucocephala

Published online by Cambridge University Press:  27 March 2009

R. Elliott ,
Janelle R. Brandon ,
G. J. Kenny  and
T. R. Evans
R. Elliott
Affiliation:
Department of Agriculture, University of Queensland, St Lucia, Brisbane, Queensland, Australia, 4067
Janelle R. Brandon
Affiliation:
Department of Agriculture, University of Queensland, St Lucia, Brisbane, Queensland, Australia, 4067
G. J. Kenny
Affiliation:
Department of Agriculture, University of Queensland, St Lucia, Brisbane, Queensland, Australia, 4067
T. R. Evans
Affiliation:
Division of Crops and Pastures, C.S.I.R.O., Cunningham Laboratory, St Lucia, Brisbane, Queensland, Australia, 4067
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Summary

Daily supplements of dried Leucaena leaf meal (500 g/head) were offered for 3 weeks to steers in metabolism cages. The steers had previously been fed diets of oaten chaff (5 kg/head/day) ormclasses and urea (4·5 kg/head/day) with restricted forage (2 kg/head/day). Excretion rates of the primary degradation product of mimosine, 3-hydroxy-4(1H) pyridone (DHP) by steers fed the two diets were compared. These compounds appeared in urine on the 1st day after Leucaena feeding had commenced and output remained fairly constant after 4 days. Mean weekly urinary outputs (g) of DHP from steers fed the oaten chaff or molasses + urea diet were 13·45 and 7·50 respectively. These levels corresponded to 0·23 and 0·13 of the amounts ingested. When steers were fed the molasses + urea diet, DHP continued to appear in urine up to 5 days after Leucaena had been withdrawn from the diet. On the oaten chaff diet no DHP was detected after 3 days following withdrawal of Leucaena.

The effects of casein or formaldehyde-treated casein supplements on the output of urinary DHP by steers fed oaten chaff plus Leucaena was evaluated in a second experiment which ran concurrently. The animals were fed the test diets for 3 weeks before collections were made. Mean DHP outputs (g/day) from animals fed the control diet, control diet plus casein or control diet plus formaldehyde-treated casein were 1·89, 2·21 and 2·44 respectively.

These results confirm substantial degradation of DHP and that the extent of degradation can be affected by the dietary regime in which Leucaena is fed.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 103 , Issue 1 , August 1984 , pp. 239 - 243
Copyright
Copyright © Cambridge University Press 1984

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References

Ferguson, K. A., Hemsley, J. A. & Reis, P. J. (1967). The effect of protecting dietary protein from microbial degradation in the rumen. Australian Journal of Science 30, 215–217.Google Scholar
Hathcock, J. N., Labadan, M. M. & Mateo, J. P. (1975). Effects of dietary protein level on toxicity of Leucaena leucocephala. Nutrition Reports International 11 (1), 55–62.Google Scholar
Hegarty, M. P., Court, R. D. & Thorne, P. M. (1964). The determination of mimosine and 3,4 dihydroxypyridone in biological material. Australian Journal of Agricultural Research 15, 168–179.CrossRefGoogle Scholar
Hegarty, M. P., Lee, C. P., Christie, G. S., Court, R. D. & Haydock, K. P. (1979). The goitrogen 3 hydroxy-4(1H) pyridone, a ruminal metabolite from Leucaena leucocephala. Effects in mice and rats. Australian Journal of Biological Sciences 32, 27–40.CrossRefGoogle ScholarPubMed
Hegarty, M. P., Schinkel, P. G. & Court, R. D. (1964). Reaction of sheep to the consumption of Leucaena glauca Benth and its toxic principle mimosine. Australian Journal of Agricultural Research 15, 153–165.CrossRefGoogle Scholar
Jones, R. J. (1979). The value of Leucaena leucocephala as a feed for ruminants in the tropics. World Animal Review 31, 13–23.Google Scholar
Jones, R. J. (1981). Does ruminal metabolism explain the absence of Leucaena toxicity in Hawaii? Australian Veterinary Journal 57, 55.Google Scholar
Jones, R. J., Blunt, C. G. & Holmes, J. H. G. (1976). Enlarged thyroid glands in cattle grazing Leucaena pastures. Tropical Grasslands 10, 113–116.Google Scholar
Jones, R. J., Blunt, C. G. & Nuenberg, B. I. (1978). The effect of iodine and mineral supplements on penned steers fed a sole diet of Leucaena. Australian Veterinary Journal 54, 387–392.Google Scholar
Lowey, J. B., Tangendjaja, M. & Tangendjaja, B. (1983). Autolysis of mimosine to 3-hydroxy-4–1(H) pyridone in green tissues of Leucaena leucocephala. Journal of the Science of Food and Agriculture 34, 529–533.Google Scholar
Matsumoto, H. & Shekman, G. D. (1951). A rapid colorimetric method for the determination of mimosine. Archives of Biochemistry and Biophysics 33, 195–200.CrossRefGoogle ScholarPubMed
Preston, T. R. & Willis, M. B. (1970). Intensive Beef Production. Oxford: Pergamon.Google Scholar
Reis, P. J. & Tunks, D. A. (1978). The influence of nutrition on the effectiveness of mimosine for defleecing sheep. Australian Journal of Agricultural Research 29, 1057–1064.Google Scholar
Tangendjaja, B., Hogan, J. P. & Wills, R. B. H. (1983). Degradation of mimosine by rumen contents: effects of feed composition and Leucaena substrates. Australian Journal of Agricultural Research 34 (2), 289–295.CrossRefGoogle Scholar