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High-temperature-adapted Azospirillum brasilense strains: growth and interaction response on associative nitrogen fixation, mineral uptake and yield of cheena (Panicum miliaceum L.) genotypes in calcareous soil

Published online by Cambridge University Press:  27 March 2009

R. Rai
R. Rai
Affiliation:
Rajendra Agricultural University, Dholi Campus, Dholi (Muzaffarpur)- 843121, Bihar, India
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Summary

High-temperature-adapted strains RAU 1, RAU 2 and RAU 3 of Azospirillum brasilense C 7 were isolated from stepwise transfer to higher temperature (30 to 42 °C). One of the strains (RAU 1) showed more growth, greater nitrogenase and hydrogenase activities at 30 and 42 °C than parental and other temperature-adapted strains. This strain also showed growth and more nitrogenase activity from pH 6·5 to 8·0. Strain RAU 1 showed cross-resistance to penicillin (300/µg/ml) but not to streptomycin, kanamycin, viomycin and polymixin B at 30 and 42 °C. It was demonstrated in field plots in calcareous soil that seed inoculation with RAU 1 enhanced mineral uptake of cheena. Inoculation with RAU 1 led to a significant increase in associative nitrogen fixation, dry weight of roots, grain and straw yield of cheena compared with the uninoculated control with or without applied N, but the effect of seed inoculation with high-temperature-adapted strains was variable with different genotypes of cheena.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 110 , Issue 2 , April 1988 , pp. 321 - 329
Copyright
Copyright © Cambridge University Press 1988

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