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Interaction between finger millet (Eleusine coracana) genotypes and drug-resistant mutants of Azospirillum brasilense in calcareous soil

Published online by Cambridge University Press:  27 March 2009

R. Rai ,
V. Prasad  and
I. C. Shukla
R. Rai
Affiliation:
Rajendra Agricultural University, Dholi Campus, Muzaffarpur-84312l, Bihar, India
V. Prasad
Affiliation:
Rajendra Agricultural University, Dholi Campus, Muzaffarpur-84312l, Bihar, India
I. C. Shukla
Affiliation:
Department of Chemistry, University of Allahabad, India
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Summary

Azospirillum brasilense was treated with nitrosoguanidine and five drug-resistant mutant strains isolated. The effects of acriflavin on pre- and post-irradiation with u.v. light and the level of antibiotic resistance were studied. Variations in factors were found between the strains. Inoculation of finger millet with A. brasilense and mutant strains led to significant increases in grain yield and nitrogenase activity compared with the uninoculated control, with significant strain x genotype interactions. Differential response of genotype and strain was noted on the protein and amino acid concentration of seeds.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 102 , Issue 3 , June 1984 , pp. 521 - 529
Copyright
Copyright © Cambridge University Press 1984

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References

Albrecht, S. L., Okon, Y. & Burris, R. H. (1977). Effect of light and temperature on the association between Zea mays and Spirillum lipoferum. Plant Physiology 60, 528–531.CrossRefGoogle ScholarPubMed
Berlier, Y. M. & Lespinate, P. A. (1980). Mass-spectrometric kinetic studies of the nitrogenase and hydrogenase activities in vivo cultures of Azospirillum brasilense sp. 7. Archives of Microbiology 125, 67–72.CrossRefGoogle Scholar
Bulow, J. F. W. & Döbereiner, J. (1975). Potential of nitrogen fixation in maize genotype in Brazil. Proceedings of the National Academy of Sciences, U.S.A. 72, 2389–2393.CrossRefGoogle Scholar
Cohen, E., Okon, Y., Kigel, J., Nur, I. & Henis, Y. (1980). Increase in dry weight and total content in Zea maysand Setaria italica associated with nitrogen-fixing Azospirillum spp. Plant Physiology 66, 746–749.Google Scholar
De-Polli, H., Boyer, C. D. & Neyra, C. A. (1982). Nitrogenase activity associated with roots and stems of field grown corn (Zea mays L.) plants. Plant Physiology 70, 1609–1613.CrossRefGoogle ScholarPubMed
Döbereiner, J. (1981). Emerging technology based on BNF by associative N2-fixing organisms. Paper presented at International Workshop on Biological Nitrogen Fixation Technology for Tropical Agriculture, 9–13 March 1981. California, Colombia.Google Scholar
Döbereiner, J. & Baldani, V. (1979). Selective infection of maize roots by steptomycin-resistant Azospirillum lipoferum and other bacteria. Canadian Journal of Microbiology 25, 1264–1269.CrossRefGoogle ScholarPubMed
Döbereiner, J. & Day, J. M. (1976). Associative symbiosis in tropical grasses: characterization of micro-organisms, and dinitrogen fixing sites. In Proceedings of the 1st International Symposmm on N2-Fixation (ed. Newton, W. E. and Nyman, C. J.), pp. 518–538. Pullman: Washington Sate University Press.Google Scholar
Döbereiner, J., Marriel, J. E. & Neyra, M. (1976). Ecological distribution of Spirillum lipoferum Beijerinck. Canadian Journal of Microbiology 22, 1464–1473.CrossRefGoogle ScholarPubMed
Emerich, D. W., Ruiz-Argüeso, T., Ching, T. N. & Evans, H. J. (1979). Hydrogen-dependent nitro-genase activity and ATP formation in Rhizobium japonicum bacteroids. Journal of Bacteriology 137, 153–160.Google Scholar
Kleckner, N., Roth, J. & Botstein, D. (1977). Genetic engineering in vivo using translocatable drug resistance elements: new methods in bacterial genetics. Journal of Molecular Biology 116, 125–159.CrossRefGoogle ScholarPubMed
Lakshmi-Kumari, M., Kavimandan, S. K. & Subba Rao, N. S. (1976). Occurrence of nitrogen fixing spirillum in roots of rice, sorghum, maize and other plants. Indian Journal of Experimental Biology 114, 638–639.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265–275.Google Scholar
Nelson, N. (1944). A photometric adaptation of the somogyi method for the determination of glucose. Journal of Biological Chemistry 153, 375–380.Google Scholar
Neyra, C. A. & DÖbereiner, J. (1977). Nitrogen fixation in grasses. Advances in Agronomy 29, 1–38.CrossRefGoogle Scholar
Okon, Y., Albrecht, S. L. & Burris, R. H. (1977). Methods for growing Spirillum lipoferum and counting it in pure culture and in association with plants. Applied Environmental Microbiology 33, 85–88.Google Scholar
Pedroantonio, A. P., Döbereiner, J. & Neyra, C. A. (1981). Nitrogen assimilation and dissimilation in five genotypes of Brachiaria spp. Canadian Journal of Botany 59, 1475–1479.Google Scholar
Rai, R. (1983). Efficacy of associative N2-fixation by streptomycin-resistant mutants of Azospirillum brasilense with genotypes of chick pea Rhizobium strains. Journal of Agricultural Science, Cambridge 100, 75–80.CrossRefGoogle Scholar
Schubert, K. R. & Evans, H. J. (1976). Hydrogen evolution: a major factor affecting the efficiency of nitrogen fixation in nodulated symbionts. Proceedings of the National Academy of Sciences 73, 1207–1211.CrossRefGoogle Scholar
Witkin, E. M. (1969). Ultraviolet induced mutation and DNA repair. Annual Review of Genetics 3, 525–552.CrossRefGoogle Scholar