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Overexpression of the Arabidopsis vacuolar H+-pyrophosphatase AVP1 gene in rice plants improves grain yield under paddy field conditions

Published online by Cambridge University Press:  14 October 2013

Y. S. KIM ,
I. S. KIM ,
Y. H. CHOE ,
M. J. BAE ,
S. Y. SHIN ,
S. K. PARK ,
H. G. KANG ,
Y. H. KIM  and
H. S. YOON
Y. S. KIM
Affiliation:
Department of Biology, Kyungpook National University, Daegu 702-701, South Korea
I. S. KIM*
Affiliation:
Department of Biology, Kyungpook National University, Daegu 702-701, South Korea
Y. H. CHOE
Affiliation:
Department of Biology, Kyungpook National University, Daegu 702-701, South Korea
M. J. BAE
Affiliation:
Department of Biology, Kyungpook National University, Daegu 702-701, South Korea
S. Y. SHIN
Affiliation:
Department of Biology, Kyungpook National University, Daegu 702-701, South Korea
S. K. PARK
Affiliation:
School of Applied Biosciences, Kyungpook National University, Daegu 702-701, South Korea
H. G. KANG
Affiliation:
Subtropical Horticulture Research Institute, Jeju National University, Jeju 690-756, South Korea
Y. H. KIM
Affiliation:
National Institute of Crop Science, Rural Development Administration, Suwon 441-857, South Korea
H. S. YOON*
Affiliation:
Department of Biology, Kyungpook National University, Daegu 702-701, South Korea
*
*To whom all correspondence should be address. Email: 92kis@hanmail.net; hyoon@knu.ac.kr
*To whom all correspondence should be address. Email: 92kis@hanmail.net; hyoon@knu.ac.kr
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Summary

The Arabidopsis gene AVP1 encodes a vacuolar H+-translocating inorganic pyrophosphatase (enzyme classification (EC) 3.6.1.1) that functions as an electronic proton pump in the vacuolar membrane and affects growth development and the stress response in plants. The current study was conducted to evaluate the molecular properties of the Arabidopsis thaliana vacuolar H+-pyrophosphatase (AVP1) gene in rice (Oryza sativa L.). Incorporation and expression of the transgene was confirmed by semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR. Expression of the AVP1 gene in transgenic rice plants (TRP1 and TRP2) resulted in a significantly enhanced tolerance to 5·8 g/l NaCl under greenhouse conditions when compared with the control wild-type (WT) rice plants. Augmented AVP1 expression in the transgenic rice plants (TRP) also affected total biomass and improved ion homoeostasis through increased accumulation of Na+ ions in whole tissues when compared with control WT rice plants under high salinity conditions. The photochemical yield (Fv/Fm) values of TRP were higher than those of the WT rice plants, even though the values decreased over time in both the WT and transgenic (TRP1 to TRP8) rice plants. Furthermore, rice grain yield and biomass of the TRP were at least 15% higher based on culm and root weights, and panicle and spikelet numbers when compared with those of the WT rice plants during the 2010 and 2010 growing seasons in South Korea. Thus, these results suggest that ectopic AVP1 expression conferred tolerance and stress resistance to genetically modified transgenic crop plants by improving cellular ion homoeostasis in response to saline conditions, which enhanced rice yield and biomass under natural conditions in paddy fields.

Type
Crops and Soils Research Papers
Information
The Journal of Agricultural Science , Volume 152 , Issue 6 , December 2014 , pp. 941 - 953
Copyright
Copyright © Cambridge University Press 2013 

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