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Culture and fertilization of sheep ovarian oocytes:II. Timing of nuclear maturation in oocytes cultured in growth medium

Published online by Cambridge University Press:  27 March 2009

T. F. Crosby  and
I. Gordon
T. F. Crosby
Affiliation:
Faculty of Agriculture, University College, Dublin
I. Gordon
Affiliation:
Faculty of Agriculture, University College, Dublin
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Summary

In 450 oocytes, recovered from the vesicular follicles of prepuberal lambs and cultured in growth medium for 2–48 h, 76·4% resumed meiosis. The first evidence of polar body extrusion was at 18 h; maturation was completed in most oocytes by 25–27 h.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 76 , Issue 3 , June 1971 , pp. 373 - 374
Copyright
Copyright © Cambridge University Press 1971

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References

REFERENCES

Berry, R. O. & Savery, H. P. (1958). A cytological study of the maturation process of the ovum of the ewe during normal and induced ovulation. Rep. & Fertil. III Symposium, pp. 75–82.Google Scholar
Dziuk, P. J. (1965). Timing of maturation and fertilization of the sheep egg. Anat. Rec. 153, 211–23.CrossRefGoogle ScholarPubMed
Quirke, J. F. & Gordon, I. (1971). Culture and fertilization of sheep ovarian oocytes. I. Effect of culture medium on resumption of meiosis. J. agric. Sci., Camb. 76, 369–72.CrossRefGoogle Scholar