Hostname: page-component-7479d7b7d-wxhwt Total loading time: 0 Render date: 2024-07-12T23:32:09.608Z Has data issue: false hasContentIssue false
  • English
  • Français

Effect of glycine on storage of ram semen

Published online by Cambridge University Press:  27 March 2009

S. I. Ahmed
S. I. Ahmed
Affiliation:
Department of Animal Husbandry, Punjab, Lahore (Pakistan) and A.R.C. Unit of Animal Reproduction, School of Agriculture, University of Cambridge
Get access Rights & Permissions [Opens in a new window]

Extract

Ram semen was stored at 4° C. in a standard citrate-egg-yolk diluent, which served as a control, and in various diluents in which: (a) the citrate buffer was replaced by various concentrations of glycine in water (2, 3, 4 and 6%); (b) the ratio of semen to diluent was altered from 1:20 to 1:40; (c) the glycine buffer was augmented by the addition of fructose; and (d) the concentration of egg yolk in the diluent was reduced from 50% to 33·3 and 25%.

Replacing citrate buffer by 4% glycine considerably improved survival as against other concentrations of glycine and the citrate buffer control. Better survival in 4% glycine-egg-yolk buffer was obtained with a semen dilution ratio of 1:20 than with a ratio of 1: 40. The addition of fructose to the glycine buffer did not improve survival. The 50% concentration of egg yolk in 4% glycine buffer-eggyolk diluent was reduced to 33·3% without affecting either motility or survival, and to 25% with no effect on survival but with slight reduction in motility.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 46 , Issue 2 , August 1955 , pp. 164 - 167
Copyright
Copyright © Cambridge University Press 1955

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bishop, M. W. H. & Salisbury, G. W. (1954). Amer. J. Physiol. (in the Press).Google Scholar
Greene, W. W. (1940). Proc. Amer. Soc. Anim. Prod. p. 207.Google Scholar
Kempschmidt, R. F., Mayer, B. T., Herman, R. A. & Dickerson, G. E. (1951). J. Dairy Sci. 34, 45.CrossRefGoogle Scholar
Philips, R. W., Fraps, R. M. & Frank, A. H. (1945). Amer. J. Vet. Res. 6, 165.Google Scholar
Roy, A. & Bishop, M. W. H. (1954). Nature, Lond., 174, 746.CrossRefGoogle Scholar
Stewart, D. L., Melrose, B. R. & Wilson, W. R. (1950). Vet. Rec. 62, 617.CrossRefGoogle Scholar
Swanson, E. W. (1949). J. Dairy Sci. 32, 345.Google Scholar
Walton, A. (1945). The Technique of Artificial Insemination, 3rd ed.London.Google Scholar