Six hundred and sixty-two sheep in twenty-two farm flocks were treated in June/July (period of late anoestrus) with intravaginal sponges impregnated with 30 mg ‘Cronolone’ (9α-fluoro-11β-hydroxy-17α-acetoxy-progesterone; G. D. Searle) or ‘Cronolone’ plus 400 mg progesterone and incorporating doses of 0-800 μg oestradiol-17β. Treatment was for 12 days; a single dose of 500 i.u. PMS (Burrough's Wellcome) was injected at pessary withdrawal. Seventy rams were joined to breed the ewes at first and second heats.

Oestradiol treatment resulted in a significantly higher incidence of sponge loss, a lower mating response, a lower conception rate and a lower number of lambs/conception. There was also a tendency for onset of oestrus to be delayed. Such effects were confined to first services and were much less evident in sheep receiving doses of 50 μg oestradiol or below. Data indicate that an intravaginal treatment for the induction of early breeding should consist of progestagen alone rather than progestagen in combination with oestradiol. The addition of 400 mg progesterone to the ‘Cronolone’ sponges appeared to reduce the adverse oestrogenic effects. Sheep treated with ‘Cronolone’/progesterone pessaries in the absence of oestradiol showed a mating response of 97%, a conception rate of 64% and 1·7 lambs/conception at the controlled oestrus.