Hostname: page-component-78c5997874-94fs2 Total loading time: 0 Render date: 2024-11-19T12:34:20.119Z Has data issue: false hasContentIssue false
  • English
  • Français

Nodule development and nitrogen fixation in cultivars of Phaseolus vulgaris L. as influenced by planting density

Published online by Cambridge University Press:  27 March 2009

P. H. Graham  and
J. C. Rosas
P. H. Graham
Affiliation:
Centro International de Agricultura Tropical, Apariado Aéreo 67–13, Call, Colombia, South America
J. C. Rosas
Affiliation:
Centro International de Agricultura Tropical, Apariado Aéreo 67–13, Call, Colombia, South America
Get access Rights & Permissions [Opens in a new window]

Summary

Three cultivars of P. vulgaris, tested 39 and 69 days after planting, differed in the extent to which nitrogen (C2H2) fixation and nodule development were affected by plant density.

At the 39-day harvest the climbing cultivar P590 declined in fixation from 15·1 μmol C2H4 produced/plant/h at 8·5 plants/m2, to only 4·2 μmol C2H4 produced/plant/h at 41·5 plants/m2; while the prostrate P498 showed consistently high fixation from 8·5 to 41·5 plants/m2. Variation in nitrogen fixation could be explained largely by change in nodule weight, though specific nodule activity (SNA) was affected, particularly in the cultivar P590. Since the percentage soluble carbohydrate in nodules of two varieties increased at certain planting densities while SNA decreased, some nodule dysfunction is possible.

Density changes affected leaf, root and stem development in all cultivars, leaves on the lower nodes being most severely affected.

At the 69-day harvest two varieties were advanced in filling the pods and showed poor nodulation and limited fixation, even at the widest plant spacings, while nodulation and nitrogen (C2H2) fixation in the third variety, which had just flowered, showed optima similar to the earlier harvest.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 90 , Issue 1 , February 1978 , pp. 19 - 29
Copyright
Copyright © Cambridge University Press 1978

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bleasdale, J. K. A. (1967). Systematic designs for spacing experiments. Experimental Agriculture 3, 73–85.CrossRefGoogle Scholar
Centro Internacional de Agricultura Tropical (1976). Annual Report for 1976.Google Scholar
Date, R. J. (1965). Legume inoculation and legume inoculant production. FAO Technical Report (1965), 2012, 33 pp.Google Scholar
Edje, O. T., Ayonoadu, U. W. U. & Mughogho, L. K. (1974). Response of indeterminate beans to varying plant populations. Turrialba 24, 100–3.Google Scholar
Edje, O. T., Mughogho, L. K. & Ayonoadu, U. W. U. (1975). Bean yield and yield components as affected by fertiliser and plant population. Turrialba 25, 79–84.Google Scholar
Goulden, D. S. (1976). Effects of plant populations and row spacing on yield and components of yield of navy beans (Phaseolus vulgaris L.). New Zealand Journal of Experimental Agriculture 19, 177–80.CrossRefGoogle Scholar
Graham, D. & Smydzuk, K. (1965). Use of anthrone in the quantitative determination of hexose phosphates. Analytical Biochemistry 11, 246–55.CrossRefGoogle ScholarPubMed
Graham, P. H. & Halliday, J. (1977). Inoculation and nitrogen fixation in the genus Phaseolus. In Exploiting the Legume Bhizobium Symbiosis in Tropical Agriculture (ed. Vincent, J. M.), Mauii 1976, pp. 313–34.Google Scholar
Graham, P. H. & Rosas, J. C. (1977). Growth and development of indeterminate bush and climbing cultivars of Phaseolus vulgaris L. inoculated with Bhizobium. Journal of Agricultural Science, Cambridge 88, 503–9.CrossRefGoogle Scholar
Hassid, W. Z. & Nueffeld, E. F. (1964). Quantitative determination of starch in plant tissues. In Methods in Carbohydrate Chemistry, vol. 4 (ed. Whistler, R. L.), pp. 33–6. New York: Academic Press.Google Scholar
Hardy, R. W. F. & Havelka, U. D. (1976). Photosynthate as a major factor limiting nitrogen fixation by field grown legumes with emphasis on soybeans. In Symbiotic Nitrogen Fixation in Plants (ed. Nutman, P. S.), pp. 421–39. Cambridge University Press.Google Scholar
Henson, I. W. & Wheeler, C. T. (1976). Hormones in plants bearing nitrogen fixing root nodules: the distribution of oytokinins in Vida faba L. New Phytologist 76, 433–9.CrossRefGoogle Scholar
Lawn, R. J. & Brun, W. A. (1974). Symbiotic nitrogen fixation in soybeans. I. Effect of photosynthetic source-sink manipulations. Crop Science 14, 11–19.CrossRefGoogle Scholar
Lawrie, A. C. & Wheeler, C. T. (1974). The effects of flowering and fruit formation on the supply of photosynthetic assimilates to the nodules of Pisum sativum L. in relation to the fixation of nitrogen. New Phytologist 73, 1119–27.CrossRefGoogle Scholar
Lucas, E. O. & Milbourn, G. M. (1976). The effect of density of planting on the growth of two Phaseolus vulgaris varieties in England. Journal of Agricultural Science, Cambridge 87, 89–99.CrossRefGoogle Scholar
McCready, R. M., Guggolz, J., Siveira, V. & Owens, H. S. (1950). Determination of starch and amylose in vegetables. Analytical Chemistry 22, 1156–8.CrossRefGoogle Scholar
Poppo, A., Rigaud, J. & Barthe, P. (1964). Sur la presence de cytokinines dans les nodules de Phaseolus vulgaris L. Comptes Rendus hebdomadaires des seances de Vacademie des sciences Ser. D. 279, 2029–32.Google Scholar
Salazar, J., Wiersma, J. & Adams, M. W. (1977). IKI-Starch status in bean varieties at three stages of seed development. Annual Report of the Bean Improvement Cooperative (1977) 20 (in the Press).Google Scholar
Sprent, J. I. (1976). Nitrogen fixation by legumes subjected to water and light stresses. In Symbiotic Nitrogen Fixation in Plants (ed. Nutman, P. S.), pp. 405–20. Cambridge University Press.Google Scholar
Tanaka, A. (1973). Studies on the physiology of Phaseolus vulgaris. Mimeographed report to the Rockefeller Foundation on work carried out in 1973, 34 pp. University of Hokkaido, Japan.Google Scholar
Viera, C. (1968). Efeitos da densidade de plantio sobre a cultura de feijoerio. Ceres 15, 45–53.Google Scholar
Yemm, E. W. & Willis, A. J. (1954). The estimation of carbohydrate in plant extracts by anthrone. Biochemistry Journal 57, 508–14.CrossRefGoogle ScholarPubMed