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Comparative study of the separation of casein from bovine, ovine and caprine milks using HPLC

Published online by Cambridge University Press:  01 June 2009

Stelios E. Kaminarides  and
Emmanuel M. Anifantakis
Stelios E. Kaminarides
Affiliation:
Agricultural University of Athens, lera Odos 75, Votanikos, 11855 Athens, Greece
Emmanuel M. Anifantakis
Affiliation:
Agricultural University of Athens, lera Odos 75, Votanikos, 11855 Athens, Greece
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Summary

The separation of cows', sheep's and goats' milk casein components by HPLC on a strong anion-exchange (P.L-SAX 8μ 1000A) column is described. During HLPC, whole caseins of the three kinds of milk behaved differently from conventional separations. The casein components of the three kinds of milk were well resolved under the Chromatographie conditions used. HPLC resolved the κ-caseins better than did ion-exchange chromatography on DEAE cellulose, and was particularly efficient in the case of goats' milk. Goats' and sheep's milks had almost similar Chromatographie profiles but these differed considerably from that of cows' milk. Caseins from the sheep and the goat were also similar in that a shallower NaCl gradient was required for the separation of casein components than for cows' milk.

Type
Original Articles
Information
Journal of Dairy Research , Volume 60 , Issue 4 , November 1993 , pp. 495 - 504
Copyright
Copyright © Proprietors of Journal of Dairy Research 1993

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