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Crude glycerol as glycogenic precursor in feed; effects on milk coagulation properties and metabolic profiles of dairy cows

Published online by Cambridge University Press:  11 March 2013

Hedi Harzia ,
Kalle Kilk ,
Tiia Ariko ,
Marko Kass ,
Ursel Soomets ,
Ivi Jõudu ,
Tanel Kaart ,
David Arney ,
Olav Kärt  and
Meelis Ots
Hedi Harzia*
Affiliation:
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 1, Tartu 51014, Estonia Bio-Competence Centre of Healthy Dairy Products, Kreutzwaldi 1, 51014 Tartu, Estonia
Kalle Kilk
Affiliation:
Department of Biochemistry, Medical Faculty, University of Tartu, the Centre of Excellence for Translational Medicine, 50411 Tartu, Estonia
Tiia Ariko
Affiliation:
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 1, Tartu 51014, Estonia
Marko Kass
Affiliation:
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 1, Tartu 51014, Estonia Bio-Competence Centre of Healthy Dairy Products, Kreutzwaldi 1, 51014 Tartu, Estonia
Ursel Soomets
Affiliation:
Department of Biochemistry, Medical Faculty, University of Tartu, the Centre of Excellence for Translational Medicine, 50411 Tartu, Estonia
Ivi Jõudu
Affiliation:
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 1, Tartu 51014, Estonia Bio-Competence Centre of Healthy Dairy Products, Kreutzwaldi 1, 51014 Tartu, Estonia
Tanel Kaart
Affiliation:
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 1, Tartu 51014, Estonia Bio-Competence Centre of Healthy Dairy Products, Kreutzwaldi 1, 51014 Tartu, Estonia
David Arney
Affiliation:
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 1, Tartu 51014, Estonia
Olav Kärt
Affiliation:
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 1, Tartu 51014, Estonia
Meelis Ots
Affiliation:
Estonian University of Life Sciences, Institute of Veterinary Medicine and Animal Sciences, Kreutzwaldi 1, Tartu 51014, Estonia Bio-Competence Centre of Healthy Dairy Products, Kreutzwaldi 1, 51014 Tartu, Estonia
*
*For correspondence; e-mail: hedi.harzia@emu.ee
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Abstract

As grain prices rise, the search for alternative glycogenic precursors in animal feed becomes increasingly important, and this study was conducted to determine if the replacement of starch with glycerol, as an alternative glycogenic precursor, affects the milk metabolic profile and milk coagulation ability, and therefore the quality of the milk. Eight primiparous mid-lactation Holstein cows were fed during a replicated 4 × 4 Latin square trial with four different isoenergetic rations: (1) control (T0) fed a total mixed ration (TMR) with barley meal; (2) group T1, decreased barley content, replaced isoenergetically with 1 kg crude glycerol; (3) group T2, the barley meal was replaced with 2 kg of crude glycerol; and (4) group T3 the barley meal was replaced with 3 kg of crude glycerol. Rumen, blood and milk samples were collected at the end of every 21-d treatment period. Rumen samples were analysed for proportion of total volatile fatty acid (VFA), blood samples for insulin and glucose, and milk for metabolites (e.g. citric-acid cycle compounds). The change in glycogenic precursors had a positive effect on rumen VFA proportions; the proportion of propionic acid increased (P < 0·001). Milk protein (P < 0·001) and curd firmness (P < 0·001) both increased. The increase in milk protein concentration may have been due to an increase in microbial protein. Regarding the milk metabolic profiles, different signals were positively associated with coagulation ability and change in the diet. Based on this study, changing the glycogenic precursor in animal diet in this way is possible, and may have no immediate deleterious consequences on milk quality or cow health. Indeed, there is evidence for benefits from this substitution.

Keywords

Glycerolglycogenic precursorsmilk coagulationmetabolomics
Type
Research Article
Information
Journal of Dairy Research , Volume 80 , Issue 2 , May 2013 , pp. 190 - 196
Copyright
Copyright © Proprietors of Journal of Dairy Research 2013

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References

Association of Official Analytical Chemists (AOAC) 2005 Official Methods of Analysis of AOAC International, 18th Edition. Gaithersburg, MD, USA: AOAC InternationalGoogle Scholar
Baticz, O, Tomoskozi, S, Vida, L & Gaal, T 2002 Relationship between concentration of citrate and ketone bodies in cow's milk. Acta Veterinaria Hungarica 50 253–261Google Scholar
Bechdel, SI, Honeywell, HE, Dutcher, RA & Knutsen, MH 1928 Synthesis of vitamin B in the rumen of the cow. Journal of Biological Chemistry 80 231–238Google Scholar
Bender, DA 2009 Pantothenic acid. In Nutritional Biochemistry of the Vitamins, pp. 341–359 (Ed. Bender, DA). Cambridge: Cambridge University PressGoogle Scholar
Bonomi, A 2000 Dairy cattle ration integration with rumen-protected pantothenic acid. Effects on milk production and reproductive efficiency. Rivista di Scienza dell'Alimentazione 29 321–338Google Scholar
Boyd, J, West, JW & Bernard, JK 2011 Effects of the addition of direct-fed microbials and glycerol to the diet of lactating dairy cows on milk yield and apparent efficiency of yield. Journal of Dairy Science 94 4616–4622Google Scholar
Cottyn, BG & Boucque, CV 1968 Rapid method for the gas-chromatographic determination of volatile fatty acids in rumen fluid. Journal of Agricultural and Food Chemistry 16 105–107CrossRefGoogle Scholar
DeFrain, JM, Hippen, AR, Kalscheur, KF & Jardon, PW 2004 Feeding glycerol to transition dairy cows: effects on blood metabolites and lactation performance. Journal of Dairy Science 87 4195–4206Google Scholar
Donkin, SS & Doane, P 2007 Glycerol as a feed ingredient in dairy rations. In Proceedings of the Tri-State Dairy Nutrition Conference, Proceedings, pp. 97–103Fort Wayne, IN: Ohio State University, ColumbusGoogle Scholar
Donkin, SS, Koser, SL, White, HM, Doane, PH & Cecava, MJ 2009 Feeding value of glycerol as a replacement for corn grain in rations fed to lactating dairy cows. Journal of Dairy Science 92 5111–5119CrossRefGoogle ScholarPubMed
Elliot, JM 1976 The glucose economy of the lactating dairy cow. In Proceedings of the Cornell Nutrition Conference for Feed Manufacturors, pp. 59–66Ithaca, NY: Cornell UniversityGoogle Scholar
Faulkner, A & Peaker, M 1982 Reviews of the progress of dairy science: secretion of citrate into milk. Journal of Dairy Research 49 159–169Google Scholar
Garnsworthy, PC, Masson, LL, Lock, AL & Mottram, TT 2006 Variation of milk citrate with stage of lactation and de novo fatty acid synthesis in dairy cows. Journal of Dairy Science 89 1604–1612CrossRefGoogle ScholarPubMed
Guinee, TP, Mulholland, EO, O'Brien, B & Murphy, JJ 2001 Effect of diet quality on the suitability of mid-lactation bovine milk for cheddar cheese manufacture. Australian Journal of Dairy Technology 56 3–8Google Scholar
Harzia, H, Kilk, K, Jõudu, I, Henno, M, Kärt, O & Soomets, U 2012 Comparison of the metabolic profiles of noncoagulating and coagulating bovine milk. Journal of Dairy Science 95 533–540Google Scholar
Hayes, BW, Mitchell, GE Jr. & Little, CO 1967 Factors affecting concentrations of B-vitamins in ruminal fluid in vitro. Internationale Zeitschrift für Vitaminforschung 37 348–353Google ScholarPubMed
Hayirli, A 2006 The role of exogenous insulin in the complex of hepatic lipidosis and ketosis associated with insulin resistance phenomenon in postpartum dairy cattle. Veterinary Research Communications 30 749–774Google Scholar
Hollis, L, Chappel, CF, MacVicar, R & Whitehair, CK 1954 Effect of ration on vitamin synthesis in rumen of sheep. Journal of Animal Science 13 732–738Google Scholar
Holt, C & Muir, DD 1979 Inorganic constituents of milk: I. Correlation of soluble calcium with citrate in bovine milk. Journal of Dairy Research 46 433–439CrossRefGoogle Scholar
Huhtanen, P, Miettinen, H & Ylinen, M 1993 Effect of increasing ruminal butyrate on milk yield and blood constituents in dairy cows fed a grass silage-based diet. Journal of Dairy Science 76 1114–1124Google Scholar
Huntington, GB 1997 Starch utilization by ruminants: from basics to the bunk. Journal of Animal Science 75 852–867CrossRefGoogle Scholar
Jõudu, I, Henno, M, Kaart, T, Püssa, T & Kärt, O 2008 The effect of milk protein contents on the rennet coagulation properties of milk from individual dairy cows. International Dairy Journal 18 964–967Google Scholar
Keogh, MK, Kelly, PM, O'Keeffe, AM & Phelan, JA 1982 Studies of milk composition and its relationship to some processing criteria: II. Seasonal variation in the mineral levels of milk. Irish Journal of Food Science and Technology 6 13–27Google Scholar
Khalili, H, Varvikko, T, Toivonen, V, Hissa, K & Suvitie, M 1997 The effects of added glycerol or unprotected free fatty acids or a combination of the two on silage intake, milk production, rumen fermentation and diet digestibility in cows given grass silage based diets. Agricultural and Food Science in Finland 6 349–362Google Scholar
Kim, C-H, Choung, J-J & Chamberlain, DG 2001 Estimates of the efficiency of transfer of L-histidine from blood to milk when it is the first-limiting amino acid for secretion of milk protein in the dairy cow. Journal of the Science of Food and Agriculture 81 1150–1155Google Scholar
Klein, MS, Buttchereit, N, Miemczyk, SP, Immervoll, AK, Louis, C, Wiedemann, S, Junge, W, Thaller, G, Oefner, PJ & Gronwald, W 2012 NMR metabolomic analysis of dairy cows reveals milk glycerophosphocholine to phosphocholine ratio as prognostic biomarker for risk of ketosis. Journal of Proteome Research 11 1373–1381Google Scholar
Kübarsepp, I, Henno, M, Kärt, O & Tupasela, T 2005 A comparison of the methods for determination of the rennet coagulation properties of milk. Acta Agriculturae Scandinavica Section a – Animal Science 55 145–148Google Scholar
Lardinois, CC, Mills, RC, Elvehjem, CA & Hart, EB 1944 Rumen Synthesis of the Vitamin B Complex as Influenced by Ration Composition. Journal of Dairy Science 27 579–583Google Scholar
Larsen, M, Lund, P, Weisbjerg, MR & Hvelplund, T 2009 Digestion site of starch from cereals and legumes in lactating dairy cows. Animal Feed Science and Technology 153 236–248Google Scholar
Linzell, JL, Mepham, TB & Peaker, M 1976 The secretion of citrate into milk. Journal of Physiology 260 739–750CrossRefGoogle ScholarPubMed
Mach, N, Bach, A & Devant, M 2009 Effects of crude glycerin supplementation on performance and meat quality of Holstein bulls fed high-concentrate diets. Journal of Animal Science 87 632–638Google Scholar
Malossini, F, Bovolenta, S, Piras, C, DallaRosa, M & Ventura, W 1996 Effect of diet and breed on milk composition and rennet coagulation properties. Annales De Zootechnie 45 29–40Google Scholar
Mitchel, LGE 1979 Seasonal variation in citrate content of milk. Australian Journal of Dairy Technology 34 158–160Google Scholar
Murphy, MR, Baldwin, RL & Koong, LJ 1982 Estimation of stoichiometric parameters for rumen fermentation of roughage and concentrate diets. Journal of Animal Science 55 411–421Google Scholar
Ørskov, ER 1986 Starch digestion and utilization in ruminants. Journal of Animal Science 63 1624–1633Google Scholar
Pathak, AK 2008 Various factors affecting microbial protein synthesis in the rumen. Veterinary World 1 186–189Google Scholar
Rigout, S, Hurtaud, C, Lemosquet, S, Bach, A & Rulquin, H 2003 Lactational effect of propionic acid and duodenal glucose in cows. Journal of Dairy Science 86 243–253Google Scholar
Rosenthal, I 1991 Milk and Dairy Products: Properties and Processing. New York: VHC PublisherGoogle Scholar
Sundekilde, UK, Frederiksen, PD, Clausen, MR, Larsen, LB & Bertram, HC 2011 Relationship between the metabolite profile and technological properties of bovine milk from two dairy breeds elucidated by NMR-based metabolomics. Journal of Agricultural and Food Chemistry 59 7360–7367CrossRefGoogle ScholarPubMed
Thomas, GB, Pearce, DT, Oldham, CM, Martin, GB & Lindsay, DR 1988 Effects of breed, ovarian steroids and season on the pulsatile secretion of LH in ovariectomized ewes. Journal of Reproduction and Fertility 84 313–324Google Scholar
Waldo, DR 1973 Extent and partition of cereal grain starch digestion in ruminants. Journal of Animal Science 37 1062–1074Google Scholar
Wang, C, Liu, Q, Yang, WZ, Huo, WJ, Dong, KH, Huang, YX, Yang, XM & He, DC 2009 Effects of glycerol on lactation performance, energy balance and metabolites in early lactation Holstein dairy cows. Animal Feed Science and Technology 151 12–20Google Scholar
Vabariiklik Söötmisalase Uurimistöö Koordineerimise Komisjon 1995 Põllumajandusloomade söötmisnormid koos söötade tabelitega (Feeding Recommendations and Nutrient tables for Farm Animals) (Eds Oll, Ü & Tölp, S). Tartu: Eesti PõllumajandusülikoolGoogle Scholar
Visser, J, Schaier, RW & van Gorkom, M 1979 The role of calcium, phosphate and citrate ions in the stabilization of casein micelles. Journal of Dairy Research 46 333–335CrossRefGoogle ScholarPubMed