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Differential leucocyte count for ewe milk with low and high somatic cell count

Published online by Cambridge University Press:  07 December 2010

Marzia Albenzio  and
Mariangela Caroprese
Marzia Albenzio*
Affiliation:
Department of Production and Innovation in Mediteranean Agriculture and Food Systems (PrIME), University of Foggia, Italy
Mariangela Caroprese
Affiliation:
Department of Production and Innovation in Mediteranean Agriculture and Food Systems (PrIME), University of Foggia, Italy
*
*For correspondence: m.albenzio@unifg.it
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Abstract

This study was undertaken to compare flow cytometry (FC) and direct microscopic leucocyte count (MDLC) for the differentiation of macrophages, lymphocytes and polymorphonuclear leucocyte (PMN) and to evaluate leucocyte distribution in ewe milk with low and high somatic cell count (SCC). Milk samples were grouped for somatic cell count in low SCC (LSCC) when the content was lower than 5·00×105/ml and high SCC (HSCC) when the content was higher than 1·00×106/ml. No differences were found between the two methods tested suggesting that FC could be used as a routine test for rapid discrimination of leucocytes. Percentages of lymphocytes in ewe milk were higher in LSCC (50%) than in HSCC (39%) and count ranged from 273·91±56·62×103cells/ml (LSCC) to 308·90±46·15×103cells/ml (HSCC). PMN number was lower in LSCC than in HSCC (248·83±46·87×103cells/ml v. 444·38±58·62×103cells/ml); accordingly the percentage was lower in LSCC (40%) than in HSCC (57%). No differences were found for macrophages which were 36·36±5·51×103cells/ml and 39·32±6·83×103cells/ml in LSCC and HSCC, respectively. Lymphocytes in ewe milk did not vary with increased number of somatic cells and were the predominant cell type in LSCC. PMN represented the main population detected in HSCC and the correlation with SCC evidenced that this leucocyte class could be useful in differentiating ewe milk cell count, being strictly responsible for the SCC increase.

Keywords

Ewe milksomatic cell countdifferential cell countflow cytometry
Type
Research Article
Information
Journal of Dairy Research , Volume 78 , Issue 1 , February 2011 , pp. 43 - 48
Copyright
Copyright © Proprietors of Journal of Dairy Research 2010

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