Hostname: page-component-5c6d5d7d68-7tdvq Total loading time: 0 Render date: 2024-09-01T11:02:07.000Z Has data issue: false hasContentIssue false
  • English
  • Français

Effects of αs1-casein (CSN1S1) and κ-casein (CSN3) genotypes on milk coagulation properties in Murciano-Granadina goats

Published online by Cambridge University Press:  10 January 2011

Francisco Caravaca ,
José Luis Ares ,
Juan Carrizosa ,
Baltasar Urrutia ,
Francisca Baena ,
Jordi Jordana ,
Bouabid Badaoui ,
Armand Sànchez ,
Antonella Angiolillo  and
Marcel Amills
...Show all authors
Francisco Caravaca
Affiliation:
Departamento de Ciencias Agroforestales, E.U.I.T.A., Universidad de Sevilla, 41013 Sevilla, Spain
José Luis Ares
Affiliation:
Instituto Andaluz de Investigación y Formación Agraria y Pesquera, Alameda del Obispo s/n, 14004 Córdoba, Spain
Juan Carrizosa
Affiliation:
Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA). Estación Sericícola. La Alberca, Murcia, Spain
Baltasar Urrutia
Affiliation:
Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA). Estación Sericícola. La Alberca, Murcia, Spain
Francisca Baena
Affiliation:
Departamento de Producción Animal, Campus de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain
Jordi Jordana
Affiliation:
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
Bouabid Badaoui
Affiliation:
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
Armand Sànchez
Affiliation:
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
Antonella Angiolillo
Affiliation:
Dip. Scienze per la Salute, via de Sanctis snc, Università del Molise, Campobasso, Italy
Marcel Amills
Affiliation:
Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain
Juan Manuel Serradilla*
Affiliation:
Departamento de Producción Animal, Campus de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain
*
*For correspondence; e-mail: pa1semaj@uco.es
Get access Rights & Permissions [Opens in a new window]

Abstract

The effects of the caprine αs1-casein (CSN1S1) polymorphisms on milk quality and cheese yield have been widely studied in French and Italian goat breeds. Much less is known about the consequences of κ-casein (CSN3) genotype on the technological and coagulation properties of goat milk. In the current study, we have performed an association analysis between polymorphisms at the goat CSN1S1 and CSN3 genes and milk coagulation (rennet coagulation time, curdling rate and curd firmness) and technological (time to cutting of curd and cheese yield) properties. In this analysis, we have included 193 records from 74 Murciano-Granadina goats (with genotypes constituted by different combinations of alleles B, E and F of the gene CSN1S1 and alleles A and B of the gene CSN3) distributed in three herds, which were collected bimonthly during a whole lactation. Data analysis, using a linear mixed model for repeated observations, revealed significant associations between CSN1S1 genotypes and the rate of the curdling process. In this way, milk from EE goats had a significantly higher curdling rate than milk from BB individuals (P<0·05). Contrary to previous experiments performed in French breeds, cheese yield was not significantly different in BB, EE and EF goats. Moreover, we have shown that CSN3 genotype has a significant effect on the rennet coagulation time (BB>AB, P<0·05) but not on cheese yield. No interaction between the CSN1S1 and CSN3 genotypes was observed.

Keywords

Goatαs1-caseinκ-caseinmilk coagulation parameters
Type
Research Article
Information
Journal of Dairy Research , Volume 78 , Issue 1 , February 2011 , pp. 32 - 37
Copyright
Copyright © Proprietors of Journal of Dairy Research 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Agüera, P, Urrutia, B, Sánchez, A, Ares, JL, Amigo, L & Serradilla, JM 2004 Near Infrared calibrations for αs1-casein fraction from goat milk. Near Infrared Spectroscopy. Proceedings of the 11th International Conference. (Ed. Davies, AMC & Garrido-Varo, A) pp. 601–604. Brighton, West Sussex, UK: NIRS PublicationsGoogle Scholar
Ambrosoli, R, DiStasio, L & Mazzocco, P 1988 Content of αs1-casein and coagulation properties in goat milk. Journal Dairy Science 71 24–28CrossRefGoogle Scholar
Ares, JL 1995 Milk sector prospecting in Andalusia: Traditional technologies and social economics aspects. PhD Thesis. University of Cordoba, SpainGoogle Scholar
Caravaca, F, Amills, M, Jordana, J, Angiolillo, A, Agüera, P, Aranda, C, Menéndez-Buxadera, A, Sánchez, A, Carrizosa, J, Urrutia, B, Sánchez, A & Serradilla, JM 2008 Effect of αs1-casein (CSN1S1) genotype on milk CSN1S1 content in Malagueña and Murciano-Granadina goats. Journal of Dairy Research 75 481–484CrossRefGoogle ScholarPubMed
Caravaca, F, Carrizosa, J, Urrutia, B, Baena, F, Jordana, J, Amills, M, Badaoui, B, Sánchez, A, Angiolillo, A & Serradilla, JM 2009 Effect of αs1-Casein (CSN1S1) and κ-Casein (CSN3) Genotypes on Milk Composition in Murciano-Granadina Goats. Journal of Dairy Science 92 2960–2964CrossRefGoogle ScholarPubMed
Caroli, A, Chiatti, F, Chessa, S, Rignanese, D, Ibeagha-Awemu, EM & Erhardt, G 2007 Characterization of the casein gene complex in West African goats and description of a new αs1-casein polymorphism. Journal of Dairy Science 90 2989–2996CrossRefGoogle Scholar
Chiatti, F, Chessa, S, Bolla, P, Cigalino, G, Caroli, A & Pagnacco, G 2007 Effect of κ-casein polymorphism on milk composition in the Orobica goat. Journal of Dairy Science 90 1962–1966CrossRefGoogle ScholarPubMed
Clark, S & Sherbon, JW 2000 Alphas1-casein, milk composition and coagulation properties of goat milk. Small Ruminant Research 38 123–134CrossRefGoogle Scholar
Colin, O, Laurent, F & Vignon, B 1992 Relations between soft cheese yield variations and milk composition or coagulation parameter variations. Lait 72 307–319CrossRefGoogle Scholar
Guyonnet, JP 1999 Optigraph: A potential substitute of Formagraph. Revue Laitiere Française 595 36–37Google Scholar
Jann, OC, Prinzenberg, EM, Luikart, G, Caroli, A & Erhardt, G 2004 High polymorphism in the κ-casein (CSN3) gene from wild and domesticated caprine species revealed by DNA sequencing. Journal of Dairy Research 71 188–195CrossRefGoogle ScholarPubMed
Kübarsepp, I, Henno, M, Kärt, O & Tupasela, T 2005 A comparison of the methods for determination of the rennet coagulation properties of milk. Acta Agriculturae Scandinavica Section A 55 145–148CrossRefGoogle Scholar
Kumar, S, Clarke, AR, Hooper, ML, Horne, DS, Law, AJ, Leaver, J, Springbett, A, Stevenson, E & Simons, JP 1994 Milk composition and lactation of beta-casein-deficient mice. Proceedings of the National Academy of Sciences of United States of America 91 6138–6142CrossRefGoogle ScholarPubMed
Littell, RC, Henry, PR & Ammerman, CB 1998 Statistical analysis of repeated measures data using SAS procedures. Journal of Animal Science 76 1216–1231CrossRefGoogle Scholar
Lucey, JA 2002 Rennet Coagulation of Milk. In Encyclopedia of Dairy Sciences (Eds. Roginski, H, Fuquay, JW, Fox, PF), pp. 286–293. Academic Press, Vol. 1CrossRefGoogle Scholar
Martin, P, Szymanowska, M, Zwierzchowski, L & Leroux, C 2002 The impact of genetic polymorphisms on the protein composition of ruminant milks. Reproduction Nutrition Development 42 433–459CrossRefGoogle ScholarPubMed
Marziali, AS & Ng-Kwai-Hang, KF 1986 Effects of milk composition and genetic polymorphisms on coagulation properties of milk. Journal of Dairy Science 69 1793–1798CrossRefGoogle Scholar
Moioli, B, D'Andrea, M & Pilla, F 2007 Candidate genes affecting sheep and goat milk quality. Small Ruminant Research 68 179–192CrossRefGoogle Scholar
Pagnacco, G & Caroli, A 1987 Effect of casein and β-lactoglobulin genotypes on renneting properties of milk. Journal of Dairy Research 54 479–485CrossRefGoogle Scholar
Panari, G, Filippi, S & Lampis, G 2002 The use of the Optigraph for the determination of the milk-clotting activity of calf rennet. Scienza e Tecnica Lattiero-Casearia 53 1 25–32Google Scholar
Pirisi, A, Colin, O, Laurent, F, Scher, J & Parmentier, M 1994 Comparison of milk composition, cheese making properties and textural characteristics of the cheese from two groups of goats with a high or low rate of αS1-casein synthesis. International Dairy Journal 4 329–345CrossRefGoogle Scholar
Politis, I & Ng-Kwai-Hang, KF 1988 Effects of somatic cell counts and milk composition on the coagulating properties of milk. Journal of Dairy Science 71 1740–1746CrossRefGoogle Scholar
Prinzenberg, EM, Gutscher, K, Chessa, S, Caroli, A & Erhardt, G 2005 Caprine κ-casein (CSN3) polymorphism: new developments of the molecular knowledge. Journal of Dairy Science 88 1490–1498CrossRefGoogle ScholarPubMed
Remeuf, F & Lenoir, J 1986 Relationship between the physicochemical characteristics of goat's milk and its rennetability. IDF Bulletin No 202 68–72Google Scholar
Remeuf, F 1993 Influence of genetic polymorphism of caprine αs1-casein on physicochemical and technological properties of goat's milk. Lait 73 549–557CrossRefGoogle Scholar
SAS Institute 2007 SAS/STAT8 User's guide: SAS online Doc. http://v8doc.sas.com/sashtml/stat/chap41/index.htmGoogle Scholar
Schaar, J, Hanson, B & Pettersson, HE 1985 Effects of genetic variants of κ-casein and β-lactoglobulin on cheese making. Journal of Dairy Research 52 429–437CrossRefGoogle Scholar
Schopen, GC, Heck, JM, Bovenhuis, H, Visker, MH, van Valenberg, HJ & van Arendonk, JA 2009 Genetic parameters for major milk proteins in Dutch Holstein-Friesians. Journal of Dairy Science 92 1182–1191CrossRefGoogle ScholarPubMed
Storry, JE, Grandison, AS, Millard, D, Owen, AJ & Ford, GD 1983 Chemical composition and coagulating properties of renneted milks from different breeds and species of ruminant. Journal of Dairy Research 50 215–229CrossRefGoogle Scholar
Vassal, L, Delacroix-Buchet, A & Bouillon, J 1994 Effect of AA, EE and FF genetic variants of alpha s1-casein from goat milk on cheese yield and sensory properties of traditional cheeses: preliminary observations. Lait 74 89–103CrossRefGoogle Scholar
Walstra, P, Jenness, R & Badings, HT 1984 Dairy Chemistry and Physics. Wiley, New York, 467 ppGoogle Scholar
Yahyaoui, MH, Angiolillo, A, Pilla, F, Sanchez, A & Folch, JM 2003 Characterization and genotyping of the caprine kappa casein variants. Journal of Dairy Science 86 2715–2720CrossRefGoogle ScholarPubMed
Zullo, A, Barone, CMA, Chianese, L, Colatruglio, P, Occidente, M & Matassino, D 2005 Protein polymorphisms and coagulation properties of Cilentana goat milk. Small Ruminant Research 58 223–230CrossRefGoogle Scholar