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Evidence for temperate bacteriophages in two strains of Lactobacillus bulgaricus

Published online by Cambridge University Press:  01 June 2009

Pierre-Jean Cluzel ,
Monique Veaux ,
Micheline Rousseau  and
Jean-Pierre Accolas
Pierre-Jean Cluzel
Affiliation:
Laboratoire de Microbiologie Laitière, INRA, 78350 Jouy-en-Josas, France
Monique Veaux
Affiliation:
Laboratoire de Microbiologie Laitière, INRA, 78350 Jouy-en-Josas, France
Micheline Rousseau
Affiliation:
Laboratoire de Microbiologie Laitière, INRA, 78350 Jouy-en-Josas, France
Jean-Pierre Accolas
Affiliation:
Laboratoire de Microbiologie Laitière, INRA, 78350 Jouy-en-Josas, France
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Summary

Lactobacillus bulgaricus strains LT1 and LT4 previously isolated from a yogurt factory were shown to be lysogenic and inducible by mitomycin C or u.v. irradiation, induction being optimal at the beginning of exponential growth. Five indicator strains (four of L. lactis, one of L. bulgaricus) were found in which the two phage lysates propagated well in a liquid medium, but formed plaques on bacterial lawns less readily. Ca2+ was required for the phage infection of the indicator strains to reach termination. Phage particles from each strain exhibited a comparable ultrastructural morphology under the electron microscope, having an isometric head, a triple collar and a noncontractile fibre-containing tail.

Type
Original Articles
Information
Journal of Dairy Research , Volume 54 , Issue 3 , August 1987 , pp. 397 - 405
Copyright
Copyright © Proprietors of Journal of Dairy Research 1987

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References

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