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The functions and mechanisms of sequence differences of DGAT1 gene on milk fat synthesis between dairy cow and buffalo

Published online by Cambridge University Press:  02 June 2020

Dinesh Bhattarai ,
Rahim Dad ,
Tesfay Worku ,
Sutong Xu ,
Farman Ullah ,
Min Zhang ,
Xianwei Liang ,
Tingxian Den ,
Mingxia Fan  and
Shujun Zhang
Dinesh Bhattarai*
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan430070, People's Republic of China
Rahim Dad
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan430070, People's Republic of China
Tesfay Worku
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan430070, People's Republic of China
Sutong Xu
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan430070, People's Republic of China
Farman Ullah
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan430070, People's Republic of China
Min Zhang
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan430070, People's Republic of China
Xianwei Liang
Affiliation:
Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning53000, People's Republic of China
Tingxian Den
Affiliation:
Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture, Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning53000, People's Republic of China
Mingxia Fan
Affiliation:
Renmin Hospital of Wuhan University, Wuhan430060, People's Republic of China
Shujun Zhang
Affiliation:
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan430070, People's Republic of China
*
Author for correspondence: Dinesh Bhattarai, Email: dbhattarai@webmail.hzau.edu.cn
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Abstract

In this research communication we describe the DGAT1 sequence and promoter region in dairy cows and buffalo and compare the activities of DGAT1 between the two species in order to increase knowledge of the cause of milk fat variation. pGL-3 basic vectors were used to construct the reporter gene. Based on the predicted promoter region, 4 truncated plasmid vectors were constructed in cow-DGAT1 and 3 plasmid vectors in buffalo-DGAT1. Each reporter plasmid was transfected into the bovine mammary epithelial cell (BMEC), 293T cell, and CHO cells to analyze the activity using Dual-Luciferase Reporter Assay System. The results show that the region between −93 to −556 bp was essential for cow promoter activity while −84 to −590 bp was essential for buffalo promoter activity revealing these regions contain core promoter. The buffalo has higher promoter activity than cow yet it was not statistically significant. Comparison of candidate mutation K232A between cow and buffalo population revealed the presence of both the allelic population in dairy cows (lysine and alanine) however, only K (lysine) allelic amino acid was found in buffalo population. The absence of the alanine allelic population from buffalo explains the higher fat content of buffalo milk.

Keywords

DGAT1K232Amilk fatplasmid vectorspromoter analysis
Type
Research Article
Information
Journal of Dairy Research , Volume 87 , Issue 2 , May 2020 , pp. 170 - 174
Copyright
Copyright © Hannah Dairy Research Foundation 2020

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