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In vitro evaluation of nanocomposites of linseed mucilage and k-carrageenan loaded with Achyrocline satureioides nanoemulsion: a gradual-release candidate of antimicrobials for the treatment of bovine mastitis

Published online by Cambridge University Press:  08 January 2024

Gabriela Tasso Pinheiro Machado ,
Roberto Gabriel Ferreira ,
Maria Beatriz Veleirinho ,
Luciana Aparecida Honorato ,
Roberta de Oliveira Ramos ,
Marcos Antônio Segatto Silva  and
Shirley Kuhnen
Gabriela Tasso Pinheiro Machado
Affiliation:
Biochemical and Natural Products Laboratory – LABINAT, Federal University of Santa Catarina, Florianópolis 88034-001, Brazil
Roberto Gabriel Ferreira
Affiliation:
Biochemical and Natural Products Laboratory – LABINAT, Federal University of Santa Catarina, Florianópolis 88034-001, Brazil
Maria Beatriz Veleirinho
Affiliation:
Biochemical and Natural Products Laboratory – LABINAT, Federal University of Santa Catarina, Florianópolis 88034-001, Brazil
Luciana Aparecida Honorato
Affiliation:
Biochemical and Natural Products Laboratory – LABINAT, Federal University of Santa Catarina, Florianópolis 88034-001, Brazil
Roberta de Oliveira Ramos
Affiliation:
Quality Control Laboratory- LABCQ, Federal University of Santa Catarina, Florianópolis 88040-900, Brazil
Marcos Antônio Segatto Silva
Affiliation:
Quality Control Laboratory- LABCQ, Federal University of Santa Catarina, Florianópolis 88040-900, Brazil
Shirley Kuhnen*
Affiliation:
Biochemical and Natural Products Laboratory – LABINAT, Federal University of Santa Catarina, Florianópolis 88034-001, Brazil
*
Corresponding author: Shirley Kuhnen; Email: shirley.kuhnen@ufsc.br
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Abstract

This research paper presents the development and evaluation of pioneering nanocomposites (NCs) based on the combination of k-carrageenan and linseed mucilage. When loaded with macela extract nanoemulsion they present an innovative approach for the sustained release of antimicrobial herbal constituents, specifically tailored for bovine mastitis treatment. The NCs, encompassing various ratios of k-carrageenan and linseed mucilage polymers (8:2, 7:3, and 5:5 w/w) with 1.25 mg of macela extract/g of gel, underwent in vitro assessment, emphasizing viscosity, degradation speed, release of herbal actives from macela nanoemulsion and antimicrobial activity. The NCs exhibited thermoreversible characteristics, transitioning from liquid at 60°C to a gel at 25°C. NCs allowed a gradual release of phenolic compounds, reaching approximately 80% of total phenolics release (w/v) within 72 h. NCs inhibited the growth of MRSA (ATCC 33592) until 8 h of incubation. No toxic effect in vitro of NCs was found on MAC-T cells. Thus, the developed materials are relevant for the treatment of bovine mastitis, especially in the dry period, and the data support future evaluations in vivo.

Keywords

Herbal activeshydrogel compositesmastitisnanocompositesnatural polymers
Type
Research Article
Information
Journal of Dairy Research , Volume 90 , Issue 4 , November 2023 , pp. 376 - 381
Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation

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