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Pattern of serum protein fractions in dairy cows during different stages of gestation and lactation

Published online by Cambridge University Press:  16 August 2011

Giuseppe Piccione ,
Vanessa Messina ,
Angelo Schembari ,
Stefania Casella ,
Claudia Giannetto  and
Daniela Alberghina
Giuseppe Piccione*
Affiliation:
Department of Experimental Sciences and Applied Biotechnology. Faculty of Veterinary Medicine. Polo Universitario Annunziata, University of Messina, Messina, Italy
Vanessa Messina
Affiliation:
Department of Experimental Sciences and Applied Biotechnology. Faculty of Veterinary Medicine. Polo Universitario Annunziata, University of Messina, Messina, Italy
Angelo Schembari
Affiliation:
Department of Experimental Sciences and Applied Biotechnology. Faculty of Veterinary Medicine. Polo Universitario Annunziata, University of Messina, Messina, Italy
Stefania Casella
Affiliation:
Department of Experimental Sciences and Applied Biotechnology. Faculty of Veterinary Medicine. Polo Universitario Annunziata, University of Messina, Messina, Italy
Claudia Giannetto
Affiliation:
Department of Experimental Sciences and Applied Biotechnology. Faculty of Veterinary Medicine. Polo Universitario Annunziata, University of Messina, Messina, Italy
Daniela Alberghina
Affiliation:
Department of Experimental Sciences and Applied Biotechnology. Faculty of Veterinary Medicine. Polo Universitario Annunziata, University of Messina, Messina, Italy
*
*For correspondence; e-mail: giuseppe.piccione@unime.it
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Abstract

In dairy cows the period of transition from late gestation to early lactation is recognized as inducing considerable metabolic adaptation. The aim of this study was to analyse modifications in serum protein values occurring during the dry and the transition period and during lactation in a group of five Holstein cows of high average milk production. For all subjects, selected on the basis of their pregnancy status, blood samples were collected at different physiological phases: dry period (−60, −30 d to calving), transition period (almost 7 d to calving, 7 d after calving), and lactation (weeks 2, 5 and 15 after calving), for a total of eight blood samples for each cow. On each blood sample total proteins and electrophoresis analysis were performed. On the data obtained, normally distributed (P<0·05, Kolmogorov-Smirnov's test), one-way Repeated Measure Analysis of Variance (ANOVA), was applied to evaluate the influence of different stages of gestation and lactation on the considered parameters. Results showed a significant effect on total proteins, α1-globulins, β-globulins, γ-globulins and albumin/globulin ratio. Most of the detected modifications were related to the transition from gestation to lactation, indicating that it is a period of great metabolic stress for cows. On the basis of the obtained results we can affirm that the pattern of serum protein fraction rn could give information about dehydration, plasma volume expansion and hepatic function occurring during the peripartum period in dairy cows.

Keywords

Serum proteinsalbuminsglobulinsdairy cowphysiological state
Type
Research Article
Information
Journal of Dairy Research , Volume 78 , Issue 4 , November 2011 , pp. 421 - 425
Copyright
Copyright © Proprietors of Journal of Dairy Research 2011

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