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Positional distribution of fatty acids in the triglyceride classes of milk fat

Published online by Cambridge University Press:  01 June 2009

Peter W. Parodi
Peter W. Parodi
Affiliation:
Fats Research Laboratory, The Butter Marketing Board, Hamilton Central, Queensland 4007, Australia
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Summary

A sample of milk fat was fractionated into high, medium and low molecular weight triglycerides by silicic acid column chromatography. Each fraction was further separated by silver ion adsorption thin-layer chromatography into triglyceride classes which were subjected to stereospecific analysis. Trans-monoene fatty acids were incorporated into triglycerides in the same manner as their cis-isomer. The specific positional distribution for fatty acids in intact milk fat triglycerides was not always maintained in the molecular weight fractions or in the various classes of unsaturation. The significance of these findings is discussed in relation to biosynthetic mechanisms for milk fat synthesis.

Type
Original Articles
Information
Journal of Dairy Research , Volume 49 , Issue 1 , February 1982 , pp. 73 - 80
Copyright
Copyright © Proprietors of Journal of Dairy Research 1982

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References

REFERENCES

Barbano, B. M. & Sherbon, J. W. 1975 Stereospecific analysis of high melting triglycerides of bovine milk fat and their biosynthetic origin. Journal of Dairy Science 58 1–8CrossRefGoogle ScholarPubMed
Blank, M. L. & Privett, O. S. 1964 Structure of milk fat triglycerides. Journal of Dairy Science 47 481–488CrossRefGoogle Scholar
Breckenridge, W. C. 1978 Stereospecific analyses of triacylglycerols. in Handbook of Lipid Research 1 197–232 (Ed. Kuksis, A.) New York: Plenum Press.Google Scholar
Brockerhoff, H. 1965 A stereospecific analysis of triglycerides. Journal of Lipid Research 6 10–15CrossRefGoogle ScholarPubMed
Christie, W. W. 1973 Lipid Analysis p. 272. Oxford: PergamonGoogle Scholar
Christie, W. W., Hunter, M. L. & Moore, J. H. 1978 The structure of the plasma triglycerides from lactating cows. New Zealand Journal of Dairy Science and Technology 13 119–120Google Scholar
Christie, W. W. & Moore, J. H. 1969 A semimicro method for the stereospecific analysis of triglycerides. Biochimica et Biophysica Acta 176 445–452CrossRefGoogle ScholarPubMed
Christie, W. W. & Moore, J. H. 1970b A comparison of the structures of triglycerides from various pig tissues. Biochimica et Biophysica Acta 210 46–56CrossRefGoogle ScholarPubMed
Christie, W. W. & Moore, J. H. 1970b The structure of egg yolk triglycerides. Biochimica et Biophysica Acta 218 83–88CrossRefGoogle ScholarPubMed
DeMan, J. M. 1961 Physical properties of milk fat. 1. Influence of chemical modification. Journal of Dairy Research 28 81–86.CrossRefGoogle Scholar
Hansen, R. P. & Shorland, F. B. 1952 Seasonal variations in fatty acid composition of New Zealand butterfat. Biochemical Journal 52 207–216CrossRefGoogle ScholarPubMed
Jenness, R. 1974 Biosynthesis and composition of milk. Journal of Investigative Dermatology 63 109–118CrossRefGoogle Scholar
Kinsella, J. E. 1972 Stearyl CoA as a precursor of oleic acid and glycerolipids in mammary microsomes from lactating bovine: possible regulatory step in milk triglyceride synthesis. Lipids 7 349–355CrossRefGoogle ScholarPubMed
Kuksis, A. 1972 Newer developments in determination of structure of glycerides and phosphoglycerides. Progress in the Chemistry of Fats and Other Lipids 12 1–163CrossRefGoogle Scholar
Kuksis, A., McCarthy, M. J. & Beveridge, J. M. R. 1964 Triglyceride composition of native and rearranged butter and coconut oils. Journal of the American Oil Chemists’ Society 41 201–205CrossRefGoogle Scholar
Kuksis, A., Marai, L. & Myher, J. J. 1973 Triglyceride structure of milk fats. Journal of the American Oil Chemists’ Society 50 193–201CrossRefGoogle ScholarPubMed
Lin, C. Y., Smith, S. & Abraham, S. 1976 Acyl specificity in triglyceride synthesis by lactating rat mammary gland. Journal of Lipid Research 17 647–656CrossRefGoogle ScholarPubMed
McCarthy, R. D. & Coccodrilli, G. D. 1975 Structure and synthesis of milk fat. XI. Effects of heparin on paths of incorporation of glucose and palmitic acid into milk fat. Journal of Dairy Science 58 164–168CrossRefGoogle ScholarPubMed
Marshall, M. O. & Knudsen, J. 1977 The specificity of l-acyl-sn-glycerol-3-phosphate acyltransferase in microsomal fractions from lactating cow mammary gland towards short, medium and long chain acyl-CoA esters. Biochimica el Biophysica Acta 489 236–241CrossRefGoogle ScholarPubMed
Marshall, M. O. & Knudsen, J. 1979 Specificity of diacylglycerol acyltransferase from bovine mammary gland, liver and adipose tissue towards acyl-CoA esters. European Journal of Biochemistry 94 93–98CrossRefGoogle ScholarPubMed
Morrison, I. M. & Hawke, J. C. 1977 Positional distribution of fatty acids in the triglycerides of bovine milk fat with elevated levels of linoleic acid. Lipids 12 1005–1011CrossRefGoogle Scholar
Parodi, P. W. 1970 Fatty acid composition of Australian butter and milk fats. Australian Journal of Dairy Technology 25 200–205Google Scholar
Parodi, P. W. 1972 Observations on the variation in fatty acid composition of milk fat. Australian Journal of Dairy Technology 27 90–94Google Scholar
Parodi, P. W. 1973 Detection of synthetic and adulterated butterfat. 4. GLC triglyceride analysis. Australian Journal of Dairy Technology 28 38–42Google Scholar
Parodi, P. W. 1979 a Stereospecific distribution of fatty acids in bovine milk fat triglycerides. Journal of Dairy Research 46 75–81CrossRefGoogle Scholar
Parodi, P. W. 1979 b Relationship between trisaturated glyceride composition and the softening point of milk fat. Journal of Dairy Research 46 633–639CrossRefGoogle Scholar
Parodi, P. W. 1980 Separation of milk fat triglycerides into classes by silver ion adsorption thin-layer chromatography. Australian Journal of Dairy Technology 35 17–22Google Scholar
Parodi, P. W. 1981 Relationship between triglyceride structure and softening point of milk fat. Journal of Dairy Research 48 131–138CrossRefGoogle Scholar
Parodi, P. W. & Dunstan, R. J. 1971 The relationship between the fatty acid composition, the softening point and the refractive index of milk fat. Australian Journal of Dairy Technology 26 29–32Google Scholar
Pitas, R. E., Sampugna, J. & Jensen, R. G. 1967 Triglyceride structure of cows’ milk fat. 1. Preliminary observations on the fatty acid composition of positions 1, 2, and 3. Journal of Dairy Science 50 1332–1336CrossRefGoogle Scholar
Shehata, A. Y., Deman, J. M. & Alexander, J. C. 1970 A simple and rapid method for the preparation of methyl esters of fat in milligram amounts for gas chromatography. Canadian Institute of Food Technology Journal 3 85–89CrossRefGoogle Scholar
Taylor, M. W. & Hawke, J. C. 1975 Structural analysis of the triacylglycerols of bovine milk fats. New Zealand Journal of Dairy Science and Technology 10 49–57Google Scholar
Woodrow, I. L. & DeMan, J. M. 1968 Distribution of trans-unsaturated fatty acids in milk fat. Biochimica et Biophysica Acta 152 472–478CrossRefGoogle ScholarPubMed