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Production of a bacteriocin active on lactate-fermenting clostridia by Lactococcus lactis subsp. lactis immobilized in coated alginate beads

Published online by Cambridge University Press:  01 June 2009

Nicla Zezza ,
Gabriella Pasini ,
Angiolella Lombardi ,
Annick Mercenier ,
Paolo Spettoli ,
Arturo Zamorani  and
Marco Paolo Nuti
Nicla Zezza
Affiliation:
Istituto Latitero Caseario e di Biotecnologie Agroalimentari, 36016 Thiene, Italia
Gabriella Pasini
Affiliation:
Dipartimento di Biotecnologie Agrarie, Università di Padova, 35131 Padova, Italia
Angiolella Lombardi
Affiliation:
Istituto Latitero Caseario e di Biotecnologie Agroalimentari, 36016 Thiene, Italia
Annick Mercenier
Affiliation:
Dipartimento di Biotecnologie Agrarie, Università di Padova, 35131 Padova, Italia
Paolo Spettoli
Affiliation:
Dipartimento di Biotecnologie Agrarie, Università di Padova, 35131 Padova, Italia
Arturo Zamorani
Affiliation:
Dipartimento di Biotecnologie Agrarie, Università di Padova, 35131 Padova, Italia
Marco Paolo Nuti
Affiliation:
Dipartimento di Biotecnologie Agrarie, Università di Padova, 35131 Padova, Italia
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Summary

We report the isolation and immobilization of a nisinogenic strain (NZ1) of Lactococcus lactis subsp. lactis, active on gas-forming lactate-fermenting clostridia responsible for late blowing of Asiago and Montasio cheeses. The bacteriocin (nisin) produced by strain NZ1 is pronase-sensitive and is released in culture media during the growth phase. Using the sensitive indicator strain Lactobacillus delbrueckii subsp. bulgaricus NCDO 1489, a rapid microtitre plate based assay was developed for quantitative determination of the bacteriocin produced by NZ1 cells, either free or immobilized in gel beads. Scanning electron microscopy of cells immobilized in calcium alginate coated beads and viable counts of the surrounding medium showed that no cell leakage occurred during a 24 h assay. The bacteriocin released from immobilized cells reached, after 5 and 24 h, concentrations comparable to that of the free cell system after 3–4 h incubation in culture media.

Type
Original Articles
Information
Journal of Dairy Research , Volume 60 , Issue 4 , November 1993 , pp. 581 - 591
Copyright
Copyright © Proprietors of Journal of Dairy Research 1993

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References

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